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Research Papers

Analyzing quantitative light scattering spectra of phantoms measured with optical coherence tomography

[+] Author Affiliations
Tasshi Dennis, Shellee D. Dyer, Andrew Dienstfrey

National Institute of Standards and Technology, 325 Broadway, Boulder, Colorado 80305

Gurpreet Singh, Paul Rice

University of Colorado, Department of Mechanical Engineering, Boulder, Colorado 80309

J. Biomed. Opt. 13(2), 024004 (April 12, 2007October 26, 2007November 06, 2007April 30, 2008). doi:10.1117/1.2899150
History: Received April 12, 2007; Revised October 26, 2007; Accepted November 06, 2007; Published April 30, 2008
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We demonstrate the ability of multiple forms of optical coherence tomography (OCT) in the frequency domain to quantitatively size scatterers. Combined with a variety of distinct phantoms, we gain insight into the measurement uncertainties associated with using scattering spectra to size scatterers. We size spherical scatterers on a surface using swept-source OCT with an analysis based on a simple slab-mode resonance model. Automating this technique, a two-dimensional (2-D) image is created by raster scanning across a surface phantom designed to have a distinct size transition to demonstrate accuracy and repeatability. We also investigate the potential of a novel sphere-nanotube structure as a quantitative calibration artifact for use in comparing measured intensity and phase scattering spectra directly to Mie theory predictions. In another experiment, we demonstrate tissue-relevant sizing of scatterers as small as 5μm on a surface by use of a Fourier domain OCT system with 280nm of bandwidth from a supercontinuum source. We perform an uncertainty analysis for our high-resolution sizing system, estimating a sizing error of 9% for measurements of spheres with a diameter of 15μm. With appropriate modifications, our uncertainty analysis has general applicability to other sizing techniques utilizing scattering spectra.

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Citation

Tasshi Dennis ; Gurpreet Singh ; Paul Rice ; Shellee D. Dyer and Andrew Dienstfrey
"Analyzing quantitative light scattering spectra of phantoms measured with optical coherence tomography", J. Biomed. Opt. 13(2), 024004 (April 12, 2007October 26, 2007November 06, 2007April 30, 2008). ; http://dx.doi.org/10.1117/1.2899150


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