Research Papers

Raman microspectroscopy for skin cancer detection in vitro

[+] Author Affiliations
Chad A. Lieber

Vanderbilt University, Department of Biomedical Engineering, Nashville, Tennessee 37235 and Children’s Hospital of Orange County, Research Institute, Orange, California 92868

Shovan K. Majumder

Vanderbilt University, Department of Biomedical Engineering, Nashville, Tennessee 37235

Dean Billheimer

Vanderbilt University Medical Center, Department of Biostatistics, Nashville, Tennessee 37232

Darrel L. Ellis

Vanderbilt University Medical Center, Department of Medicine, Division of Dermatology, Nashville, Tennessee 37232

Anita Mahadevan-Jansen

Vanderbilt University, Department of Biomedical Engineering, Nashville, Tennessee 37232

J. Biomed. Opt. 13(2), 024013 (March 24, 2008). doi:10.1117/1.2899155
History: Received August 07, 2007; Revised October 30, 2007; Accepted November 06, 2007; Published March 24, 2008
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We investigate the potential of near-infrared Raman microspectroscopy to differentiate between normal and malignant skin lesions. Thirty-nine skin tissue samples consisting of normal, basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and melanoma from 39 patients were investigated. Raman spectra were recorded at the surface and at 20-μm intervals below the surface for each sample, down to a depth of at least 100μm. Data reduction algorithms based on the nonlinear maximum representation and discrimination feature (MRDF) and discriminant algorithms using sparse multinomial logistic regression (SMLR) were developed for classification of the Raman spectra relative to histopathology. The tissue Raman spectra were classified into pathological states with a maximal overall sensitivity and specificity for disease of 100%. These results indicate the potential of using Raman microspectroscopy for skin cancer detection and provide a clear rationale for future clinical studies.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Chad A. Lieber ; Shovan K. Majumder ; Dean Billheimer ; Darrel L. Ellis and Anita Mahadevan-Jansen
"Raman microspectroscopy for skin cancer detection in vitro", J. Biomed. Opt. 13(2), 024013 (March 24, 2008). ; http://dx.doi.org/10.1117/1.2899155


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