Research Papers

Multispectral optical imaging device for in vivo detection of oral neoplasia

[+] Author Affiliations
Darren Roblyer, Rebecca Richards-Kortum

Rice University, Department of Bioengineering, 6100 Main St., Houston, Texas 77251-1892

Konstantin Sokolov

University of Texas M. D. Anderson Cancer Center, Departments of Imaging Physics and Biomedical Engineering, 1515 Holcombe Boulevard, Houston, Texas 77030

Adel K. El-Naggar, Michelle D. Williams

University of Texas M. D. Anderson Cancer Center, Department of Pathology, 1515 Holcombe Blvd., Box 0085, Houston, Texas 77030

Cristina Kurachi, Ann M. Gillenwater

University of Texas M.D. Anderson Cancer Center, Department of Head and Neck Surgery, 1515 Holcombe Boulevard, Unit 441, Houston, Texas 77030

J. Biomed. Opt. 13(2), 024019 (May 01, 2008). doi:10.1117/1.2904658
History: Received July 26, 2007; Revised November 19, 2007; Accepted December 10, 2007; Published May 01, 2008
Text Size: A A A

A multispectral digital microscope (MDM) is designed and constructed as a tool to improve detection of oral neoplasia. The MDM acquires in vivo images of oral tissue in fluorescence, narrow-band (NB) reflectance, and orthogonal polarized reflectance (OPR) modes, to enable evaluation of lesions that may not exhibit high contrast under standard white light illumination. The device rapidly captures image sequences so that the diagnostic value of each modality can be qualitatively and quantitatively evaluated alone and in combination. As part of a pilot clinical trial, images are acquired from normal volunteers and patients with precancerous and cancerous lesions. In normal subjects, the visibility of vasculature can be enhanced by tuning the reflectance illumination wavelength and polarization. In patients with histologically confirmed neoplasia, we observe decreased blue/green autofluorescence and increased red autofluorescence in lesions, and increased visibility of vasculature using NB and OPR imaging. The perceived lesion borders change with imaging modality, suggesting that multimodal imaging has the potential to provide additional diagnostic information not available using standard white light illumination or by using a single imaging mode alone.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Darren Roblyer ; Rebecca Richards-Kortum ; Konstantin Sokolov ; Adel K. El-Naggar ; Michelle D. Williams, et al.
"Multispectral optical imaging device for in vivo detection of oral neoplasia", J. Biomed. Opt. 13(2), 024019 (May 01, 2008). ; http://dx.doi.org/10.1117/1.2904658


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.