0
Research Papers

Quantitative phase microscopy through differential interference imaging

[+] Author Affiliations
Sharon V. King, Ariel Libertun, Rafael Piestun, Carol J. Cogswell

University of Colorado, Department of Electrical and Computer Engineering, Campus Box 425 UCB Boulder, Colorado 80309-0425

Chrysanthe Preza

The University of Memphis, Department of Electrical and Computer Engineering, 206 Engineering Science Bldg Memphis, Tennessee 38152-3180

J. Biomed. Opt. 13(2), 024020 (April 21, 2008). doi:10.1117/1.2907328
History: Received August 15, 2007; Revised November 26, 2007; Accepted November 29, 2007; Published April 21, 2008
Text Size: A A A

An extension of Nomarski differential interference contrast microscopy enables isotropic linear phase imaging through the combination of phase shifting, two directions of shear, and Fourier space integration using a modified spiral phase transform. We apply this method to simulated and experimentally acquired images of partially absorptive test objects. A direct comparison of the computationally determined phase to the true object phase demonstrates the capabilities of the method. Simulation results predict and confirm results obtained from experimentally acquired images.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Sharon V. King ; Ariel Libertun ; Rafael Piestun ; Carol J. Cogswell and Chrysanthe Preza
"Quantitative phase microscopy through differential interference imaging", J. Biomed. Opt. 13(2), 024020 (April 21, 2008). ; http://dx.doi.org/10.1117/1.2907328


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Tables

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


Buy this article ($18 for members, $25 for non-members).
Sign In