Research Papers

Quantitative phase microscopy through differential interference imaging

[+] Author Affiliations
Sharon V. King, Ariel Libertun, Rafael Piestun, Carol J. Cogswell

University of Colorado, Department of Electrical and Computer Engineering, Campus Box 425 UCB Boulder, Colorado 80309-0425

Chrysanthe Preza

The University of Memphis, Department of Electrical and Computer Engineering, 206 Engineering Science Bldg Memphis, Tennessee 38152-3180

J. Biomed. Opt. 13(2), 024020 (April 21, 2008). doi:10.1117/1.2907328
History: Received August 15, 2007; Revised November 26, 2007; Accepted November 29, 2007; Published April 21, 2008
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An extension of Nomarski differential interference contrast microscopy enables isotropic linear phase imaging through the combination of phase shifting, two directions of shear, and Fourier space integration using a modified spiral phase transform. We apply this method to simulated and experimentally acquired images of partially absorptive test objects. A direct comparison of the computationally determined phase to the true object phase demonstrates the capabilities of the method. Simulation results predict and confirm results obtained from experimentally acquired images.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Sharon V. King ; Ariel Libertun ; Rafael Piestun ; Carol J. Cogswell and Chrysanthe Preza
"Quantitative phase microscopy through differential interference imaging", J. Biomed. Opt. 13(2), 024020 (April 21, 2008). ; http://dx.doi.org/10.1117/1.2907328


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