Research Papers

Measurements of light scattering in an integrated microfluidic waveguide cytometer

[+] Author Affiliations
Xuan-Tao Su, Kirat Singh

University of Alberta, Department of Physics, Edmonton, T6G 2G7, Canada

Clarence Capjack

University of Alberta, Department of Electrical & Computer Engineering, Edmonton, T6G 2V4, Canada

Jiří Petráček

Brno University of Technology, Institute of Physical Engineering, Technická 2, 61669 Brno, Czech Republic

Christopher Backhouse

University of Alberta, Department of Electrical & Computer Engineering, Edmonton, T6G 2V4, Canada

Wojciech Rozmus

University of Alberta, Department of Physics, Edmonton, T6G 2G7, Canada

J. Biomed. Opt. 13(2), 024024 (April 28, 2008). doi:10.1117/1.2909670
History: Received April 23, 2007; Revised November 26, 2007; Accepted November 26, 2007; Published April 28, 2008
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An integrated microfluidic planar optical waveguide system for measuring light scattered from a single scatterer is described. This system is used to obtain 2D side-scatter patterns from single polystyrene microbeads in a fluidic flow. Vertical fringes in the 2D scatter patterns are used to infer the location of the 90-deg scatter (polar angle). The 2D scatter patterns are shown to be symmetrical about the azimuth angle at 90deg. Wide-angle comparisons between the experimental scatter patterns and Mie theory simulations are shown to be in good agreement. A method based on the Fourier transform analysis of the experimental and Mie simulation scatter patterns is developed for size differentiation.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Xuan-Tao Su ; Kirat Singh ; Clarence Capjack ; Jiří Petráček ; Christopher Backhouse, et al.
"Measurements of light scattering in an integrated microfluidic waveguide cytometer", J. Biomed. Opt. 13(2), 024024 (April 28, 2008). ; http://dx.doi.org/10.1117/1.2909670


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