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Research Papers

Ex vivo multiphoton analysis of rabbit corneal wound healing following conductive keratoplasty

[+] Author Affiliations
Tsung-Jen Wang

Taipei Medical University Hospital, Department of Ophthalmology, Taipei 110, Taiwan and National Taiwan University Hospital, Department of Ophthalmology, Taipei 110, Taiwan and National Taiwan University, College of Medicine, Taipei 100, Taiwan

Wen Lo, Chiu Mei Hsueh

National Taiwan University, Department of Physics, Taipei 106, Taiwan

Ming-Shium Hsieh

Department of Medicine, Taipei Medical Univeristy, Taipei 110, Taiwan

Chen-Yuan Dong

National Taiwan University, Department of Physics, Taipei 106, Taiwancydong@phys.ntu.edu.tw

Fung-Rong Hu

National Taiwan University Hospital, Department of Ophthalmology, Taipei 100, Taiwan and National Taiwan University, College of Medicine, Taipei 100, Taiwan

J. Biomed. Opt. 13(3), 034019 (July 01, 2008). doi:10.1117/1.2943156
History: Received May 24, 2007; Revised December 19, 2007; Accepted December 19, 2007; Published July 01, 2008
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Ex vivo multiphoton imaging is used to characterize rabbit corneal wound healing after conductive keratoplasty (CK) procedures. CK is performed on the right eyes from eight New Zealand albino rabbits while the left eyes are punctured by a keratoplast tip without energy application. Rabbits are humanely sacrificed 1day, 1, 2, and 4weeks after the CK procedure. Eye balls are enucleated and placed on the microscope for multiphoton imaging. Multiphoton imaging reveals damage of corneal epithelium and stroma caused by the CK procedure and the subsequent wound healing process can be followed without histological procedures. Multiphoton excited autofluorescence images demonstrate that re-epithelilialization is accomplished within 1week in both CK and control groups. However, epithelial hyperplasia is observed in CK corneas. In addition, stromal wounds in the control group become inconspicuous within 1week while obvious wounds still exist in CK corneas for at least 4weeks. Postconductive keratoplasty corneal damage and wound healing can be characterized by multiphoton microscopy without histological procedures. Our results suggest that multiphoton microscopy has potential in the clinical evaluation of corneal damage due to refractive surgery, and can be used to study and reduce the unwanted side effects of these procedures.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Tsung-Jen Wang ; Wen Lo ; Chiu Mei Hsueh ; Ming-Shium Hsieh ; Chen-Yuan Dong, et al.
"Ex vivo multiphoton analysis of rabbit corneal wound healing following conductive keratoplasty", J. Biomed. Opt. 13(3), 034019 (July 01, 2008). ; http://dx.doi.org/10.1117/1.2943156


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