Special Section on Selected Topics in Biophotonics: Diffuse Optics and Optical Molecular Imaging

Molecular imaging with optics: primer and case for near-infrared fluorescence techniques in personalized medicine

[+] Author Affiliations
Eva M. Sevick-Muraca, John C. Rasmussen

Baylor College of Medicine, Department of Radiology, Division of Molecular Imaging, Houston, Texas 77030

J. Biomed. Opt. 13(4), 041303 (July 24, 2008). doi:10.1117/1.2953185
History: Received November 17, 2007; Revised February 20, 2008; Accepted March 17, 2008; Published July 24, 2008
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We compare and contrast the development of optical molecular imaging techniques with nuclear medicine with a didactic emphasis for initiating readers into the field of molecular imaging. The nuclear imaging techniques of gamma scintigraphy, single-photon emission computed tomography, and positron emission tomography are first briefly reviewed. The molecular optical imaging techniques of bioluminescence and fluorescence using gene reporter/probes and gene reporters are described prior to introducing the governing factors of autofluorescence and excitation light leakage. The use of dual-labeled, near-infrared excitable and radio-labeled agents are described with comparative measurements between planar fluorescence and nuclear molecular imaging. The concept of time-independent and -dependent measurements is described with emphasis on integrating time-dependent measurements made in the frequency domain for 3-D tomography. Finally, we comment on the challenges and pro-gress for translating near-infrared (NIR) molecular imaging agents for personalized medicine.

© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Eva M. Sevick-Muraca and John C. Rasmussen
"Molecular imaging with optics: primer and case for near-infrared fluorescence techniques in personalized medicine", J. Biomed. Opt. 13(4), 041303 (July 24, 2008). ; http://dx.doi.org/10.1117/1.2953185


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