Special Section on Selected Topics in Biophotonics: Diffuse Optics and Optical Molecular Imaging

Oxygenated hemoglobin diffuse reflectance ratio for in vivo detection of oral pre-cancer

[+] Author Affiliations
Rupananda Mallia, Shiny Sara Thomas

Centre for Earth Science Studies, Biophotonics Laboratory, Trivandrum, India

Anitha Mathews

Regional Cancer Centre, Department of Cytopathology, Trivandrum, India

Rejnish Kumar

Regional Cancer Centre, Department of Radiation Oncology, Trivandrum, India

Paul Sebastian

Regional Cancer Centre, Division of Surgical Oncology, Trivandrum, India

Jayaprakash Madhavan

Regional Cancer Centre, Department of Radiotherapy, Trivandrum, India

Narayanan Subhash

Centre for Earth Science Studies, Biophotonics Laboratory, Trivandrum, India

J. Biomed. Opt. 13(4), 041306 (July 09, 2008). doi:10.1117/1.2952007
History: Received September 04, 2007; Revised December 24, 2007; Accepted January 04, 2008; Published July 09, 2008
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Diffuse reflectance (DR) spectroscopy is a simple, low-cost, and noninvasive modality with potential for distinguishing oral precancer. Recently, in an ex vivo study, the DR spectral ratio (R545/R575) of oxygenated hemoglobin bands at 545 and 575nm was used for grading malignancy. This work presents the results of clinical trials conducted in 29 patients to detect oral precancer using this ratio. We use site-specific normal spectra from a group of 36 healthy volunteers for comparison with those of patients. Toward this, in vivo DR spectra from 14 anatomical sites of the oral cavity of healthy volunteers are recorded on a miniature fiber optic spectrometer with white light excitation. The R545/R575 ratio is lowest for healthy tissues and appears to increase with the grade of malignancy. As compared to scatter plots that use the mean DR ratio from all anatomical sites, those using site-specific data show improved sensitivity and specificity for early diagnosis and grading of oral cancer. In the case of buccal mucosa, using scatter plots of R545/R575 ratio, we obtain a sensitivity of 100% and specificity of 86% for discriminating precancer (dysplasia) from hyperplasia, and a sensitivity of 97% and specificity of 86% for discriminating hyperplasia from normal.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Rupananda Mallia ; Shiny Sara Thomas ; Anitha Mathews ; Rejnish Kumar ; Paul Sebastian, et al.
"Oxygenated hemoglobin diffuse reflectance ratio for in vivo detection of oral pre-cancer", J. Biomed. Opt. 13(4), 041306 (July 09, 2008). ; http://dx.doi.org/10.1117/1.2952007


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