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Special Section on Selected Topics in Biophotonics: Diffuse Optics and Optical Molecular Imaging

Optical properties of mutant versus wild-type mouse skin measured by reflectance-mode confocal scanning laser microscopy (rCSLM)

[+] Author Affiliations
Ravikant Samatham, Steven L. Jacques

Oregon Health & Science University, Department of Biomedical Engineering, 3303 SW Bond Avenue, Portland, Oregon 97239

Paul Campagnola

University of Connecticut Health Center, Center for Biomedical Imaging Technology, Department of Physiology, 263 Farmington Avenue, Farmington, Connecticut 06030

J. Biomed. Opt. 13(4), 041309 (September 25, 2007April 15, 2008April 28, 2008July 18, 2008). doi:10.1117/1.2953195
History: Received September 25, 2007; Revised April 15, 2008; Accepted April 28, 2008; Published July 18, 2008
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Separation of the two optical scattering properties, the scattering coefficient (μs) and the anisotropy of scattering (g), has been experimentally difficult in tissues. A new method for measuring these properties in tissues uses reflectance-mode confocal scanning laser microscopy (rCSLM). Experimentally, the focus at depth z is scanned down into the tissue. The measured data is the exponential decay of the confocal reflectance signal as a function of the depth of the focal volume, R(z)=ρexp(μz), summarized as a local reflectivity (ρ) and an exponential decay constant (μ). The ρ and μ map uniquely into the μs and g of the tissue. The method was applied to three mouse skin tissues: one wild-type (wt/wt), one heterozygous mutant (oim/wt), and one homozygous mutant (oim/oim), where oim indicates the mutation for osteogenesis imperfecta, a bone disease that affects type I collagen structure. The mutation affects the collagen fibrils of the skin and the assembly of collagen fiber bundles. The anisotropy of scattering (g) at 488 nm wavelength decreased from 0.81 to 0.46 with the added mutant allele. There was a slight increase in the scattering coefficient (μs) with the mutation from 74to94cm1. The decrease in g (toward more isotropic scattering) is likely due to the failure of the mutant fibrils to assemble into the larger collagen fiber bundles that yield forward scattering.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Ravikant Samatham ; Paul Campagnola and Steven L. Jacques
"Optical properties of mutant versus wild-type mouse skin measured by reflectance-mode confocal scanning laser microscopy (rCSLM)", J. Biomed. Opt. 13(4), 041309 (September 25, 2007April 15, 2008April 28, 2008July 18, 2008). ; http://dx.doi.org/10.1117/1.2953195


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