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Special Section on Selected Topics in Biophotonics: Diffuse Optics and Optical Molecular Imaging

Near infrared fluorescent optical imaging for nodal staging

[+] Author Affiliations
Lakshmi Sampath

Baylor College of Medicine, Division of Molecular Imaging, Department of Radiology and Molecular Physiology and Biophysics and The Baylor Breast Center, Houston, Texas 77030

Wei Wang

Baylor College of Medicine, Division of Molecular Imaging, Department of Radiology, Houston, Texas 77030

Eva M. Sevick-Muraca

Baylor College of Medicine, Division of Molecular Imaging, Department of Radiology and Molecular Physiology and Biophysics and The Baylor Breast Center, Houston, Texas 77030

J. Biomed. Opt. 13(4), 041312 (July 16, 2008). doi:10.1117/1.2953498
History: Received September 29, 2007; Revised May 04, 2008; Accepted May 08, 2008; Published July 16, 2008
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Current techniques to assess lymph node metastases in cancer patients include lymphoscintigraphy after administration of a nonspecific radiocolloid in order to locate and resect lymph nodes for pathological examination of harbored cancer cells. Clinical trials involving intradermal or subcutaneous injection of antibody-based nuclear imaging agents have demonstrated the feasibility for target-specific, molecular imaging of cancer-positive lymph nodes. The basis for employing near-infrared (NIR) optical imaging for assessing disease is evidenced by recent work showing functional lymph imaging in mice, swine, and humans. We review antibody-based immunolymphoscintigraphy with an emphasis on the use of trastuzumab (or Herceptin) to target human epidermal growth factor receptor-2 (HER2) overexpressed in some breast cancers. Specifically, we review in vitro and preclinical imaging data from our laboratory that show how the dual-labeled agent (In-DTPA111)n-trastuzumab-(IRDye800)m utilizes the high photon count provided by an NIR fluorescent dye, IRDye 800CW, and the radioactive signal from a gamma emitter, Indium-111, for possible detection of HER2 metastasis in lymph nodes. We show that the accumulation and clearance of (In-DTPA111)n-trastuzumab-(IRDye800)m from the axillary nodes of mice occurs 48h after intradermal injection into the dorsal aspect of the foot. The requirement for long clearance times from normal, cancer-negative nodes presents challenges for nuclear imaging agents with limited half-lives but does not hamper NIR optical imaging.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Lakshmi Sampath ; Wei Wang and Eva M. Sevick-Muraca
"Near infrared fluorescent optical imaging for nodal staging", J. Biomed. Opt. 13(4), 041312 (July 16, 2008). ; http://dx.doi.org/10.1117/1.2953498


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