Special Section on Selected Topics in Biophotonics: Diffuse Optics and Optical Molecular Imaging

Risk estimation of skin damage due to ultrashort pulsed, focused near-infrared laser irradiation at 800nm

[+] Author Affiliations
Frank Fischer

Research Microscopy, Beiersdorf AG, Unnastrasse 48, D-20245 Hamburg, Germany

Beate Volkmer

Dermatologisches Zentrum Buxtehude, Am Krankenhaus 1, D-21614 Buxtehude, Germany

Stefan Puschmann

Research Microscopy, Beiersdorf AG, Unnastrasse 48, D-20245 Hamburg, Germany

Ruediger Greinert, Wolfgang Breitbart

Dermatologisches Zentrum Buxtehude, Am Krankenhaus 1, D-21614 Buxtehude, Germany

Juergen Kiefer

University Giessen, Strahlenzentrum, Leihgesterner Weg 217, D-35392 Giessen, German

Roger Wepf

Research Microscopy, Beiersdorf AG, Unnastrasse 48, D-20245 Hamburg, Germany

J. Biomed. Opt. 13(4), 041320 (August 27, 2008). doi:10.1117/1.2960016
History: Received July 18, 2007; Revised April 18, 2008; Accepted May 13, 2008; Published August 27, 2008
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New imaging techniques using near-infrared (NIR) femtosecond lasers (fs-lasers) in multiphoton laser scanning microscopy (MPLSM) have great potential for in vivo applications, particularly in human skin. However, little is known about possible risks. In order to evaluate the risk, a “biological dosimeter” was used. We irradiated fresh human skin samples with both an fs-laser and a solar simulator UV source (SSU). DNA damage introduced in the epidermis was evaluated using fluorescent antibodies against cyclobutane-pyrimidin-dimers (CPDs) in combination with immunofluorescence image analysis. Four fs-irradiation regimes (at 800-nm wavelength) were evaluated differing in laser power and step width of horizontal scans. Fs-irradiation did not give CPDs at 15-mW or 30-mW irradiation power using 10 horizontal scans every 5microns. CPDs could be seen at 60-mW laser power and 5-μm step size and at 35-mW using 1-micron step width. Quantitative comparison of SSU-induced CPDs showed that the 60-mW laser irradiation regime is comparable to UV-irradiation, giving 0.6 minimal erythemal dose (MED). The 1-micron irradiation regime was comparable to 0.45 MED. Under these experimental conditions, the risk of DNA damage due to fs-laser irradiation on skin is in the range of natural UV-exposure.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Frank Fischer ; Beate Volkmer ; Stefan Puschmann ; Ruediger Greinert ; Wolfgang Breitbart, et al.
"Risk estimation of skin damage due to ultrashort pulsed, focused near-infrared laser irradiation at 800nm", J. Biomed. Opt. 13(4), 041320 (August 27, 2008). ; http://dx.doi.org/10.1117/1.2960016


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