Research Papers

Simultaneous multispectral reflectance imaging and laser speckle flowmetry of cerebral blood flow and oxygen metabolism in focal cerebral ischemia

[+] Author Affiliations
Phill B. Jones

Massachusetts General Hospital, Harvard Medical School, Martinos Center for Biomedical Imaging, and MassGeneral Institute for Neurodegenerative Disorders, Charlestown, Massachusetts 02129

Hwa Kyoung Shin

Massachusetts General Hospital, Harvard Medical School, Department of Radiology, Stroke and Neurovascular Regulation Laboratory, Charlestown, Massachusetts 02129

David A. Boas

Massachusetts General Hospital, Harvard Medical School, Martinos Center for Biomedical Imaging, Charlestown, Massachusetts 02129

Bradley T. Hyman

Massachusetts General Hospital, Harvard Medical School, MassGeneral Institute for Neurodegenerative Disorders, Charlestown, Massachusetts 02129

Michael A. Moskowitz

Massachusetts General Hospital, Harvard Medical School, Department of Radiology, Stroke and Neurovascular Regulation Laboratory, Charlestown, Massachusetts 02129

Cenk Ayata

Massachusetts General Hospital, Harvard Medical School, Department of Radiology, Stroke and Neurovascular Regulation Laboratory, and Department of Neurology, Stroke Service and Neuroscience Intensive Care Unit, Charlestown, Massachusetts 02129

Andrew K. Dunn

University of Texas, Biomedical Engineering Department, Austin Texas 78712

J. Biomed. Opt. 13(4), 044007 (July 24, 2008). doi:10.1117/1.2950312
History: Received August 13, 2007; Revised February 06, 2008; Accepted February 12, 2008; Published July 24, 2008
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Real-time investigation of cerebral blood flow (CBF), and oxy- and deoxyhemoglobin concentration (HbO, HbR) dynamics has been difficult until recently due to limited spatial and temporal resolution of techniques like laser Doppler flowmetry and magnetic resonance imaging (MRI). The combination of laser speckle flowmetry (LSF) and multispectral reflectance imaging (MSRI) yields high-resolution spatiotemporal maps of hemodynamic and metabolic changes in response to functional cortical activation. During acute focal cerebral ischemia, changes in HbO and HbR are much larger than in functional activation, resulting in the failure of the Beer-Lambert approximation to yield accurate results. We describe the use of simultaneous LSF and MSRI, using a nonlinear Monte Carlo fitting technique, to record rapid changes in CBF, HbO, HbR, and cerebral metabolic rate of oxygen (CMRO2) during acute focal cerebral ischemia induced by distal middle cerebral artery occlusion (dMCAO) and reperfusion. This technique captures CBF and CMRO2 changes during hemodynamic and metabolic events with high temporal and spatial resolution through the intact skull and demonstrates the utility of simultaneous LSF and MSRI in mouse models of cere-brovascular disease.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Phill B. Jones ; Hwa Kyoung Shin ; David A. Boas ; Bradley T. Hyman ; Michael A. Moskowitz, et al.
"Simultaneous multispectral reflectance imaging and laser speckle flowmetry of cerebral blood flow and oxygen metabolism in focal cerebral ischemia", J. Biomed. Opt. 13(4), 044007 (July 24, 2008). ; http://dx.doi.org/10.1117/1.2950312


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