Research Papers

Multispectral confocal microendoscope for in vivo and in situ imaging

[+] Author Affiliations
Houssine Makhlouf, Arthur F. Gmitro, Anthony A. Tanbakuchi, Josh A. Udovich, Andrew R. Rouse

University of Arizona, Department of Radiology, P. O. Box 245067, Tucson, Arizona 85724

J. Biomed. Opt. 13(4), 044016 (July 10, 2008). doi:10.1117/1.2950313
History: Received November 27, 2007; Revised February 03, 2008; Accepted February 06, 2008; Published July 10, 2008
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We describe the design and operation of a multispectral confocal microendoscope. This fiber-based fluorescence imaging system consists of a slit-scan confocal microscope coupled to an imaging catheter that is designed to be minimally invasive and allow for cellular level imaging in vivo. The system can operate in two imaging modes. The grayscale mode of operation provides high resolution real-time in vivo images showing the intensity of fluorescent signal from the specimen. The multispectral mode of operation uses a prism as a dispersive element to collect a full multispectral image of the fluorescence emission. The instrument can switch back and forth nearly instantaneously between the two imaging modes (less than half a second). In the current configuration, the multispectral confocal microendoscope achieves 3-μm lateral resolution and 30-μm axial resolution. The system records light from 500to750nm, and the minimum resolvable wavelength difference varies from 2.9to8.3nm over this spectral range. Grayscale and multispectral imaging results from ex-vivo human tissues and small animal tissues are presented.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Houssine Makhlouf ; Arthur F. Gmitro ; Anthony A. Tanbakuchi ; Josh A. Udovich and Andrew R. Rouse
"Multispectral confocal microendoscope for in vivo and in situ imaging", J. Biomed. Opt. 13(4), 044016 (July 10, 2008). ; http://dx.doi.org/10.1117/1.2950313


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