Research Papers

Retinal vessel oximetry-calibration, compensation for vessel diameter and fundus pigmentation, and reproducibility

[+] Author Affiliations
Martin Hammer

University of Jena, Department of Ophthalmology, Jena, Germany

Walthard Vilser, Thomas Riemer

Imedos GmbH, Jena, Germany

Dietrich Schweitzer

University of Jena, Department of Ophthalmology, Jena, Germany

J. Biomed. Opt. 13(5), 054015 (September 05, 2008). doi:10.1117/1.2976032
History: Received December 20, 2007; Revised April 08, 2008; Accepted April 30, 2008; Published September 05, 2008
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The purpose of this study was to measure the hemoglobin oxygenation in retinal vessels and to evaluate the sensitivity and reproducibility of the measurement. Using a fundus camera equipped with a special dual wavelength transmission filter and a color charge-coupled device camera, two monochromatic fundus images at 548 and 610nm were recorded simultaneously. The optical densities of retinal vessels for both wavelengths and their ratio, which is known to be proportional to the oxygen saturation, were calculated. From 50-deg images, the used semiautomatic vessel recognition and tracking algorithm recognized and measured vessels of 100μm or more in diameter. On average, arterial and venous oxygen saturations were measured at 98±10.1% and 65±11.7%, respectively. For measurements in the same vessel segments from the five images per subject, standard deviations of 2.52% and 3.25% oxygen saturation were found in arteries and veins, respectively. Respiration of 100% oxygen increased the mean arterial and venous oxygen saturation by 2% and 7% respectively. A simple system for noninvasive optical oximetry, consisting of a special filter in a fundus camera and software, was introduced. It is able to measure the oxygen saturation in retinal branch vessels with reproducibility and sensitivity suitable for clinical investigations.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Martin Hammer ; Walthard Vilser ; Thomas Riemer and Dietrich Schweitzer
"Retinal vessel oximetry-calibration, compensation for vessel diameter and fundus pigmentation, and reproducibility", J. Biomed. Opt. 13(5), 054015 (September 05, 2008). ; http://dx.doi.org/10.1117/1.2976032


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