Research Papers

Velocity distributions of single F-actin trajectories from a fluorescence image series using trajectory reconstruction and optical flow mapping

[+] Author Affiliations
Frederic von Wegner, Tobias Ober, Cornelia Weber, Sebastian Schürmann, René Winter, Oliver Friedrich, Rainer H. A. Fink

University of Heidelberg, Institute of Physiology and Pathophysiology, Medical Biophysics Group, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany

Martin Vogel

University of Heidelberg, Institute of Physiology and Pathophysiology, Medical Biophysics Group, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany and Harvard University, Center for Nanoscale Systems, 11 Oxford Street, Cambridge, Massachusetts, USA

J. Biomed. Opt. 13(5), 054018 (September 23, 2008). doi:10.1117/1.2982525
History: Received November 07, 2007; Revised May 03, 2008; Accepted May 05, 2008; Published September 23, 2008
Text Size: A A A

We present an approach for the computation of single-object velocity statistics in a noisy fluorescence image series. The algorithm is applied to molecular imaging data from an in vitro actin-myosin motility assay. We compare the relative efficiency of wavelet and curvelet transform denoising in terms of noise reduction and object restoration. It is shown that while both algorithms reduce background noise efficiently, curvelet denoising restores the curved edges of actin filaments more reliably. Noncrossing spatiotemporal actin trajectories are unambiguously identified using a novel segmentation scheme that locally combines the information of 2-D and 3-D segmentation. Finally, the optical flow vector field for the image sequence is computed via the 3-D structure tensor and mapped to the segmented trajectories. Using single-trajectory statistics, the global velocity distribution extracted from an image sequence is decomposed into the contributions of individual trajectories. The technique is further used to analyze the distribution of the x and y components of the velocity vectors separately, and it is shown that directed actin motion is found in myosin extracts from single skeletal muscle fibers. The presented approach may prove helpful to identify actin filament subpopulations and to analyze actin-myosin interaction kinetics under biochemical regulation.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Frederic von Wegner ; Tobias Ober ; Cornelia Weber ; Sebastian Schürmann ; René Winter, et al.
"Velocity distributions of single F-actin trajectories from a fluorescence image series using trajectory reconstruction and optical flow mapping", J. Biomed. Opt. 13(5), 054018 (September 23, 2008). ; http://dx.doi.org/10.1117/1.2982525


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.