Research Papers

Endoscopic image analysis of photosensitizer fluorescence as a promising noninvasive approach for pathological grading of bladder cancer in situ

[+] Author Affiliations
James Chen Yong Kah

National University of Singapore, Division of Bioengineering, 7 Engineering Drive 1, Singapore 117574

Weber Kam On Lau

Singapore General Hospital, Department of Urology, Outram Road, Singapore 169608

Phuay Hoon Tan

Singapore General Hospital, Department of Pathology, Outram Road, Singapore 169608

Colin James Richard Sheppard

National University of Singapore, Division of Bioengineering, 7 Engineering Drive 1, Singapore 117574

Malini Olivo

National Cancer Centre Singapore, Division of Medical Sciences, 11 Hospital Drive Singapore 169610 and Singapore Bioimaging Consortium, Bio-optical Imaging, 11 Biopolis Street #02-02 Helios, Singapore 138667 and National University of Singapore, Department of Pharmacy, Block S4, 18 Science Drive 4, Singapore 117543

J. Biomed. Opt. 13(5), 054022 (September 22, 2008). doi:10.1117/1.2981827
History: Received February 03, 2008; Revised April 29, 2008; Accepted May 09, 2008; Published September 22, 2008
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Our aim is to apply image analysis on photosensitizer fluorescence and compare the relationship between histopathology and endoscopic fluorescence imaging. The correlation between hypericin fluorescence and histopathology of diseased tissue was explored in a clinical study involving 58 fluorescence cystoscopic images from 23 patients. Based on quantification of fluorescence colorimetric parameters extracted from the image analysis, diagnostic functions were developed to pathologically classify the bladder cancer. Our preliminary results show that the differences in fluorescence intensity ratios among the three different grades of bladder cancer are statistically significant. The results also show a decrease in macroscopic fluorescence intensity that correlated with higher cancer grades. By combining both the red-to-green and red-to-blue fluorescence intensity ratios into a 2-D scatter plot and defining diagnostic linear discrimination functions on the data points, this technique is able to yield an average sensitivity and specificity of around 68.6% and 86.1%, respectively, for pathological cancer grading of the three different grades of bladder cancer in our study. We conclude that our proposed approach in applying colorimetric intensity ratio analysis on hypericin fluorescence shows potential to optically grade bladder cancer in situ.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

James Chen Yong Kah ; Weber Kam On Lau ; Phuay Hoon Tan ; Colin James Richard Sheppard and Malini Olivo
"Endoscopic image analysis of photosensitizer fluorescence as a promising noninvasive approach for pathological grading of bladder cancer in situ", J. Biomed. Opt. 13(5), 054022 (September 22, 2008). ; http://dx.doi.org/10.1117/1.2981827


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