Research Papers

Polymer-based microfluidics with surface-enhanced Raman-spectroscopy-active periodic metal nanostructures for biofluid analysis

[+] Author Affiliations
Kiang Wei Kho

National Cancer Centre, 11 Hospital Drive, No. 05-05, Singapore, 169610 and National University of Singapore, Department of Physics, Singapore, 117542

Kristin Zhu Mei Qing

Nanyang Technological University, School of Material Science and Engineering, Singapore, 639798

Ze Xiang Shen

Nanyang Technological University of Singapore, School of Physical and Mathematical Sciences, Singapore 639798

Iman Binte Ahmad, Samanta Sing Chin Lim, Subodh Mhaisalkar, Timothy John White

Nanyang Technological University, School of Material Sciences and Engineering, Singapore, 639798

Frank Watt

National University of Singapore, Department of Physics, Singapore, 117542

Kee Chee Soo

National Cancer Centre, 11 Hospital Drive, No. 05-05, Singapore 169610

Malini Olivo

National Cancer Centre, 11 Hospital Drive, No. 05-05, Singapore, 169610 and National University of Singapore, Department of Pharmacy, Block S4, 18 Science Drive, 4, Singapore, 117543 and Biomedical Sciences Institute, Singapore Bioimaging Consortium, 11 Biopolis Way, No. 02-02 Helios, Singapore, 138667

J. Biomed. Opt. 13(5), 054026 (September 11, 2008). doi:10.1117/1.2976140
History: Received December 02, 2007; Revised May 18, 2008; Accepted May 20, 2008; Published September 11, 2008
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The use of microfluidics for biofluid analysis offers a cheaper alternative to conventional techniques in disease diagnosis. However, traditional microfluidics design may be complicated by the need to incorporate separation elements into the system in order to facilitate specific molecular detection. Alternatively, an optical technique known as surface-enhanced Raman spectroscopy (SERS) may be used to enable identification of analyte molecules directly from a complex sample. This will not only simplify design but also reduce overall cost. The concept of SERS-based microfluidics is however not new and has been demonstrated previously by mixing SERS-active metal nanoparticles with a model sample, in situ, within the microchannel. Although the SERS reproducibility of these systems was shown to be acceptable, it is, however, not stable toward variations in the salt content of the sample, as will be shown in this study. We have proposed a microfluidics design whereby periodic SERS-active metal nanostructures are fabricated directly into the microchannel via a simple method of spin coating. Using artificial as well as human urine samples, we show that the current microfluidics is more stable toward variations in the sample’s ionic strength.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Kiang Wei Kho ; Kristin Zhu Mei Qing ; Ze Xiang Shen ; Iman Binte Ahmad ; Samanta Sing Chin Lim, et al.
"Polymer-based microfluidics with surface-enhanced Raman-spectroscopy-active periodic metal nanostructures for biofluid analysis", J. Biomed. Opt. 13(5), 054026 (September 11, 2008). ; http://dx.doi.org/10.1117/1.2976140


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