Research Papers

Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array

[+] Author Affiliations
Liang Song, Konstantin Maslov

Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St. Louis, Missouri 63130

Rachel Bitton, K. Kirk Shung

University of Southern California, Department of Biomedical Engineering, Los Angeles, California 90089

Lihong V. Wang

Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St. Louis, Missouri 63130

J. Biomed. Opt. 13(5), 054028 (September 11, 2008). doi:10.1117/1.2976141
History: Received February 28, 2008; Revised May 22, 2008; Accepted May 23, 2008; Published September 11, 2008
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We present an in vivo dark-field reflection-mode photoacoustic microscopy system that performs cross-sectional (B-scan) imaging at 50Hz with real-time beamforming and 3-D imaging consisting of 166 B-scan frames at 1Hz with postbeamforming. To our knowledge, this speed is currently the fastest in photoacoustic imaging. A custom-designed light delivery system is integrated with a 30-MHz ultrasound linear array to realize dark-field reflection-mode imaging. Linear mechanical scanning of the array produces 3-D images. The system has axial, lateral, and elevational resolutions of 25, 70, and 200μm, respectively, and can image 3mm deep in scattering biological tissues. Volumetric images of subcutaneous vasculature in rats are demonstrated in vivo. Fast 3-D photoacoustic microscopy is anticipated to facilitate applications of photoacoustic imaging in biomedical studies that involve dynamics and clinical procedures that demand immediate diagnosis.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Liang Song ; Konstantin Maslov ; Rachel Bitton ; K. Kirk Shung and Lihong V. Wang
"Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array", J. Biomed. Opt. 13(5), 054028 (September 11, 2008). ; http://dx.doi.org/10.1117/1.2976141


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