Research Papers

Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin

[+] Author Affiliations
Dan Fu

Princeton University, Department of Chemistry, Princeton, New Jersey 08544

Tong Ye, Thomas E. Matthews

Duke University, Department of Chemistry, Durham, North Carolina 27708

James Grichnik

Duke University, Department of Medicine, Durham, North Carolina 27710

Lian Hong, John D. Simon

Duke University, Department of Chemistry, Durham, North Carolina 27708

Warren S. Warren

Duke University, Department of Chemistry and Radiology, Durham, North Carolina 27708

J. Biomed. Opt. 13(5), 054036 (September 12, 2008). doi:10.1117/1.2976424
History: Received February 21, 2008; Revised May 30, 2008; Accepted June 04, 2008; Published September 12, 2008
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As some of the most ubiquitous and biologically important natural pigments, melanins play essential roles in the photoprotection of skin. Changes in melanin production could potentially be useful for clinical diagnosis of the progression stage of melanoma. Previously we demonstrated a new method for imaging melanin distribution in tissue with two-color transient absorption microscopy. Here we extend this study to longer wavelengths and show that we are able to image melanin in fixed thin skin slices with higher signal-to-noise ratios (SNRs) and demonstrate epimode imaging. We show that both photothermal effects and long-lived excited states can contribute to the long-lived signal. Eumelanin and pheomelanin exhibit markedly different long-lived excited state absorption. This difference should enable us to map out their respective distribution in tissue samples with subcellular resolution. This technique could provide valuable information in diagnosing the malignant transformation of melanocytes.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Dan Fu ; Tong Ye ; Thomas E. Matthews ; James Grichnik ; Lian Hong, et al.
"Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin", J. Biomed. Opt. 13(5), 054036 (September 12, 2008). ; http://dx.doi.org/10.1117/1.2976424


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