Research Papers

Manipulation of mammalian cells using a single-fiber optical microbeam

[+] Author Affiliations
Samarendra K. Mohanty

University of California-Irvine, Beckman Laser Institute, Irvine, California 92612

Khyati S. Mohanty, Michael W. Berns

University of California-Irvine, Beckman Laser Institute, Irvine, California 92612 and University of California-Irvine, Department of Biomedical Engineering, Irvine, California 92612

J. Biomed. Opt. 13(5), 054049 (October 14, 2008). doi:10.1117/1.2983663
History: Received December 27, 2007; Revised July 02, 2008; Accepted July 10, 2008; Published October 14, 2008
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The short working distance of microscope objectives has severely restricted the application of optical micromanipulation techniques at larger depths. We show the first use of fiber-optic tweezers toward controlled guidance of neuronal growth cones and stretching of neurons. Further, by mode locking, the fiber-optic tweezers beam was converted to fiber-optic scissors, enabling dissection of neuronal processes and thus allowing study of the subsequent response of neurons to localized injury. At high average powers, lysis of a three-dimensionally trapped cell was accomplished.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Samarendra K. Mohanty ; Khyati S. Mohanty and Michael W. Berns
"Manipulation of mammalian cells using a single-fiber optical microbeam", J. Biomed. Opt. 13(5), 054049 (October 14, 2008). ; http://dx.doi.org/10.1117/1.2983663


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