Research Papers

Second-harmonic generation in collagen as a potential cancer diagnostic parameter

[+] Author Affiliations
Tord Hompland, Arne Erikson, Mikael Lindgren, Tore Lindmo, Catharina de Lange Davies

The Norwegian University of Science and Technology, Department of Physics, Høgskoleringen 5, 7491 Trondheim, Norway

J. Biomed. Opt. 13(5), 054050 (October 14, 2008). doi:10.1117/1.2983664
History: Received May 08, 2008; Revised July 02, 2008; Accepted July 10, 2008; Published October 14, 2008
Text Size: A A A

The fibrillar collagen network in tumor and normal tissues is different due to remodeling of the extracellular matrix during the malignant process. Collagen type I fibers have the crystalline and noncentrosymmetric properties required for generating the second-harmonic signal. The content and structure of collagen were studied by imaging the second-harmonic generation (SHG) signal in frozen sections from three tumor tissues, osteosarcoma, breast carcinoma, and melanoma, and were compared with corresponding normal tissues, bone/femur, breast, and dermis/skin. The collagen density was measured as the percentage of pixels containing SHG signal in tissue images, and material parameters such as the second-order nonlinear optical susceptibility given by the d22 coefficient and an empirical anisotropy parameter were used to characterize the collagen structure. Generally, normal tissues had much more collagen than tumor tissues. In tumor tissues, a cap of collagen was seen at the periphery, and further into the tumors, the distribution of collagen was sparse and heterogeneous. The difference in structure was reflected in the two times higher d22 coefficient and lower anisotropy values in normal tissues compared with tumor tissues. Together, the differences in the collagen content, distribution, and structure show that collagen signature is a promising diagnostic marker.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Tord Hompland ; Arne Erikson ; Mikael Lindgren ; Tore Lindmo and Catharina de Lange Davies
"Second-harmonic generation in collagen as a potential cancer diagnostic parameter", J. Biomed. Opt. 13(5), 054050 (October 14, 2008). ; http://dx.doi.org/10.1117/1.2983664


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.