Research Papers

Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers

[+] Author Affiliations
Tim J. Harvey, Elsa Correia Faria, Alex Henderson

University of Manchester, School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester, Manchester M1 7DN, United Kingdom

Ehsan Gazi

University of Manchester, Christie Hospital NHS Trust, Paterson Institute for Cancer Research, Genito-Urinary Cancer Research Group, Withington, Manchester M20 4BX, United Kingdom

Andrew D. Ward

Science and Technology Facilities Council, Rutherford Appleton Laboratory, Central Laser Facility, Didcot, Oxfordshire OX11 OPX, United Kingdom

Noel W. Clarke, Michael D. Brown

University of Manchester, Christie Hospital NHS Trust, Paterson Institute for Cancer Research, Genito-Urinary Cancer Research Group, Withington, Manchester M20 4BX, United Kingdom

Richard D. Snook, Peter Gardner

University of Manchester, School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester, Manchester M1 7DN, United Kingdom

J. Biomed. Opt. 13(6), 064004 (November 05, 2008). doi:10.1117/1.2999609
History: Received December 14, 2007; Revised August 01, 2008; Accepted August 23, 2008; Published November 05, 2008
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An investigation into the use of Raman optical tweezers to study urological cell lines is reported, with the ultimate aim of determining the presence of malignant CaP cells in urine and peripheral fluids. To this end, we trapped and analyzed live CaP cells (PC-3) and bladder cells (MGH-U1), because both prostate and bladder cells are likely to be present in urine. The laser excitation wavelength of 514.5nm was used, with Raman light collected both in back- and forward-scattering geometric configurations. For the backscattering configuration the same laser was used for trapping and excitation, while for forward scattering a 1064nm laser provided the trapping beam. Analysis of cell-diameter distributions for cells analyzed suggested normal distribution of cell sizes, indicating an unbiased cell-selection criterion. Principal components analysis afforded discrimination of MGH-U1 and PC-3 spectra collected in either configuration, demonstrating that it is possible to trap, analyze, and differentiate PC-3 from MGH-U1 cells using a 514.5nm laser. By loading plot analysis, possible biomolecules responsible for discrimination in both configurations were determined. Finally, the effect of cell size on discrimination was investigated, with results indicating that separation is based predominantly on cell type rather than cell size.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Tim J. Harvey ; Elsa Correia Faria ; Alex Henderson ; Ehsan Gazi ; Andrew D. Ward, et al.
"Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers", J. Biomed. Opt. 13(6), 064004 (November 05, 2008). ; http://dx.doi.org/10.1117/1.2999609


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