Research Papers

Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy

[+] Author Affiliations
Terry A. Gaige, Hyuk Sang Kwon, Guangping Dai, Victor C. Cabral, Ruopeng Wang, Yoon Sung Nam, Bevin P. Engelward, Van J Wedeen, Peter T. C. So, Richard J. Gilbert

Massachusetts Institute of Technology, Department of Mechanical and Biological Engineering, Cambridge, Massachusetts 02139 and Massachusetts General Hospital, Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, Massachusetts 02129

J. Biomed. Opt. 13(6), 064005 (January 05, 2009). doi:10.1117/1.3046724
History: Received January 18, 2008; Revised August 09, 2008; Accepted August 24, 2008; Published January 05, 2009
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The tongue consists of a complex, multiscale array of myofibers that comprise the anatomical underpinning of lingual mechanical function. 3-D myoarchitecture was imaged in mouse tongues with diffusion spectrum magnetic resonance imaging (DSI) at 9.4T (bmax7000smm, 150-μm isotropic voxels), a method that derives the preferential diffusion of water/voxel, and high-throughput (10fps) two-photon microscope (TPM). Net fiber alignment was represented for each method in terms of the local maxima of an orientational distribution function (ODF) derived from the local diffusion (DSI) and 3-D structural autocorrelation (TPM), respectively. Mesoscale myofiber tracts were generated by alignment of the principal orientation vectors of the ODFs. These data revealed a consistent relationship between the properties of the respective ODFs and the virtual superimposition of the distributed mesoscale myofiber tracts. The identification of a mesoscale anatomical construct, which specifically links the microscopic and macroscopic spatial scales, provides a method for relating the orientation and distribution of cells and subcellular components with overall tissue morphology, thus contributing to the development of multiscale methods for mechanical analysis.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Terry A. Gaige ; Hyuk Sang Kwon ; Guangping Dai ; Victor C. Cabral ; Ruopeng Wang, et al.
"Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy", J. Biomed. Opt. 13(6), 064005 (January 05, 2009). ; http://dx.doi.org/10.1117/1.3046724


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