Research Papers

Monte Carlo model to describe depth selective fluorescence spectra of epithelial tissue: applications for diagnosis of oral precancer

[+] Author Affiliations
Ina Pavlova

Cornell University, School of Applied and Engineering Physics, Ithaca, New York 14853

Crystal Redden Weber

Rice University, Department of Chemistry, Houston, Texas 77005

Richard A. Schwarz

Rice University, Department of Bioengineering, Houston, Texas 77005

Michelle Williams, Adel El-Naggar

The University of Texas M. D. Anderson Cancer Center, Department of Pathology, Houston, Texas 77030

Ann Gillenwater

The University of Texas M. D. Anderson Cancer Center, Department of Head and Neck Surgery, Houston, Texas 77030

Rebecca Richards-Kortum

Rice University, Department of Bioengineering, Houston, Texas 77005

J. Biomed. Opt. 13(6), 064012 (November 05, 2008). doi:10.1117/1.3006066
History: Received November 06, 2007; Revised August 27, 2008; Accepted August 28, 2008; Published November 05, 2008
Text Size: A A A

We present a Monte Carlo model to predict fluorescence spectra of the oral mucosa obtained with a depth-selective fiber optic probe as a function of tissue optical properties. A model sensitivity analysis determines how variations in optical parameters associated with neoplastic development influence the intensity and shape of spectra, and elucidates the biological basis for differences in spectra from normal and premalignant oral sites. Predictions indicate that spectra of oral mucosa collected with a depth-selective probe are affected by variations in epithelial optical properties, and to a lesser extent, by changes in superficial stromal parameters, but not by changes in the optical properties of deeper stroma. The depth selective probe offers enhanced detection of epithelial fluorescence, with 90% of the detected signal originating from the epithelium and superficial stroma. Predicted depth-selective spectra are in good agreement with measured average spectra from normal and dysplastic oral sites. Changes in parameters associated with dysplastic progression lead to a decreased fluorescence intensity and a shift of the spectra to longer emission wavelengths. Decreased fluorescence is due to a drop in detected stromal photons, whereas the shift of spectral shape is attributed to an increased fraction of detected photons arising in the epithelium.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Ina Pavlova ; Crystal Redden Weber ; Richard A. Schwarz ; Michelle Williams ; Adel El-Naggar, et al.
"Monte Carlo model to describe depth selective fluorescence spectra of epithelial tissue: applications for diagnosis of oral precancer", J. Biomed. Opt. 13(6), 064012 (November 05, 2008). ; http://dx.doi.org/10.1117/1.3006066


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.