Research Papers

Photoacoustic monitoring of burn healing process in rats

[+] Author Affiliations
Kazuya Aizawa

Keio University, Department of Electronics and Electrical Engineering, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan and Japan Self Defense Force Central Hospital, 1–2–24, Ikejiri, Setagaya-ku, Tokyo 154–8532, Japan

Shunichi Sato

National Defense Medical College Research Institute, Division of Biomedical Information Sciences, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

Daizoh Saitoh

National Defense Medical College Research Institute, Division of Basic Traumatology, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

Hiroshi Ashida

National Defense Medical College Research Institute, Division of Biomedical Information Sciences, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

Minoru Obara

Keio University, Department of Electronics and Electrical Engineering, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan

J. Biomed. Opt. 13(6), 064020 (November 26, 2008). doi:10.1117/1.3028005
History: Received December 26, 2007; Revised July 23, 2008; Accepted September 05, 2008; Published November 26, 2008
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We performed multiwavelength photoacoustic (PA) measurement for extensive deep dermal burns in rats to monitor the healing process of the wounds. The PA signal peak at 532nm, an isosbestic point for oxyhemoglobin (HbO2) and deoxyhemoglobin (HHb), was found to shift to a shallower region of the injured skin tissue with the elapse of time. The results of histological analysis showed that the shift of the PA signal reflected angiogenesis in the wounds. Until 24h postburn, PA signal amplitude generally increased at all wavelengths. We speculate that this increase in amplitude is associated with dilation of blood vessels within healthy tissue under the injured tissue layer and increased hematocrit value due to development of edema. From 24to48h postburn, the PA signal showed wavelength-dependent behaviors; signal amplitudes at 532, 556, and 576nm continued to increase, while amplitude at 600nm, an HHb absorption-dominant wavelength, decreased. This seems to reflect change from shock phase to hyperdynamic state in the rat; in the hyperdynamic state, cardiac output and oxygen consumption increased considerably. These findings show that multiwavelength PA measurement would be useful for monitoring recovery of perfusion and change in local hemodynamics in the healing process of burns.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Kazuya Aizawa ; Shunichi Sato ; Daizoh Saitoh ; Hiroshi Ashida and Minoru Obara
"Photoacoustic monitoring of burn healing process in rats", J. Biomed. Opt. 13(6), 064020 (November 26, 2008). ; http://dx.doi.org/10.1117/1.3028005


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