Research Papers

Intrinsic fluorescence spectroscopy for endoscopic detection and localization of the endobronchial cancerous lesions

[+] Author Affiliations
Yasser Fawzy

Perceptronix Medical, Incorporated, Suite 400, 555 W. 8th Avenue, Vancouver, BC V5Z 1C6 Canada and National Institute of Laser Enhanced Sciences (NILES), Cairo University, Cairo, Egypt

Haishan Zeng

Cancer Imaging Department, BC Cancer Agency, 675 W. 10th Avenue, Vancouver, BC V5Z 1L3 Canada

J. Biomed. Opt. 13(6), 064022 (December 16, 2008). doi:10.1117/1.3041704
History: Received January 25, 2008; Revised October 02, 2008; Accepted October 06, 2008; Published December 16, 2008
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Fluorescence spectroscopy contains diagnostic information about the lung biochemistry and morphology, including tissue optical properties and fluorophores. However, the fluorophore information is generally masked by the optical properties of the tissue, which complicates the evaluation of their role in lung-cancer detection. In this work, we have developed a method for extracting the intrinsic fluorescence spectra from the endoscopic measurements of the combined fluorescence and reflectance spectra. Principle components and classification analysis was performed to evaluate the diagnostic potential of the extracted intrinsic fluorescence spectra from in vivo combined fluorescence and reflectance spectral measurements. We evaluated the diagnostic sensitivity and specificity of both the intrinsic fluorescence and the fluorescence spectra. The results showed that the intrinsic fluorescence spectra contain significant diagnostic information that had been masked by the lung optical properties. We have also found that the intrinsic fluorescence has improved the specificity for endobronchial-cancer detection, although with a slight decrease in the detection sensitivity, when compared to the fluorescence spectra. This may indicate that intrinsic fluorescence analysis could be used to improve the diagnostic specificity of fluorescence spectroscopy and imaging.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Yasser Fawzy and Haishan Zeng
"Intrinsic fluorescence spectroscopy for endoscopic detection and localization of the endobronchial cancerous lesions", J. Biomed. Opt. 13(6), 064022 (December 16, 2008). ; http://dx.doi.org/10.1117/1.3041704


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