Research Papers

In vivo staining of neocortical astrocytes via the cerebral microcirculation using sulforhodamine B

[+] Author Affiliations
Pascale Vérant

Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5588, Laboratoire de Spectrométrie Physique, Grenoble, F-38402 France and Université Joseph Fourier, Grenoble, F-38041 France and Institut Fresnel, Equipe Mosaic, Marseille, F-13397 France

Clément Ricard, Raphaël Serduc

Université Joseph Fourier, Grenoble, F-38041 France and Institut National de la Santé et de la Recherche Médicale, U836, Grenoble Institut des Neurosciences, Grenoble, F-38043 France

Jean-Claude Vial

Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5588, Laboratoire de Spectrométrie Physique, Grenoble, F-38402 France and Université Joseph Fourier, Grenoble, F-38041 France

Boudewijn van der Sanden

Institut National de la Santé et de la Recherche Médicale, U836, Grenoble Institut des Neurosciences, Grenoble, F-38043 France

J. Biomed. Opt. 13(6), 064028 (December 15, 2008). doi:10.1117/1.3041163
History: Received January 08, 2008; Revised October 10, 2008; Accepted October 13, 2008; Published December 15, 2008
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Staining and imaging glial cells in vivo while observing the microvasculature could help understand brain physiology, namely neuronal-glial-vascular communication. Two-photon excitation microscopy provides a means to monitor these interactions at the cellular level in living animals, but the cells of interest must be fluorescent. Injecting dyes intravenously is a rapid and quasi noninvasive method to stain cells in the brain. It necessitates that the dye is soluble in the blood plasma and crosses the blood brain barrier (BBB). We demonstrate here, using two-photon imaging, that sulforhodamine B (SRB) crosses the BBB and stains in vivo, specifically mouse astrocytes. This is confirmed by experiments on primary neurons and astrocytes cultures showing the preferential SRB staining of the latter. SRB is rapidly eliminated from the blood, which allows repeated injections in longitudinal studies.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Topics

Blood ; Brain ; Nerve ; Photons ; Plasmas ; Water

Citation

Pascale Vérant ; Clément Ricard ; Raphaël Serduc ; Jean-Claude Vial and Boudewijn van der Sanden
"In vivo staining of neocortical astrocytes via the cerebral microcirculation using sulforhodamine B", J. Biomed. Opt. 13(6), 064028 (December 15, 2008). ; http://dx.doi.org/10.1117/1.3041163


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