Research Papers

Model-based spectroscopic analysis of the oral cavity: impact of anatomy

[+] Author Affiliations
Sasha McGee, Jelena Mirkovic

Massachusetts Institute of Technology, G.R. Harrison Spectroscopy Laboratory, Building 6-205, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139

Vartan Mardirossian, Alphi Elackattu

Boston Medical Center, 4th Floor, FGH Building, 820 Harrison Avenue, Boston, Massachusetts 02118

Chung-Chieh Yu

Massachusetts Institute of Technology, G.R. Harrison Spectroscopy Laboratory, Building 6-205, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139

Sadru Kabani, George Gallagher

Boston University Goldman School of Dental Medicine, Department of Oral and Maxillofacial Pathology, 100 East Newton Street G-04, Boston, Massachusetts 02118

Robert Pistey

Boston Medical Center, Department of Anatomic Pathology, 670 Albany Street, 3rd floor (BIO-3), Boston, Massachusetts 02118

Luis Galindo

Massachusetts Institute of Technology, G.R. Harrison Spectroscopy Laboratory, Building 6-205, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139

Kamran Badizadegan

Harvard Medical School and Massachusetts General Hospital, Department of Pathology, 55 Fruit Street, WRN219, Boston, Massachusetts 02114

Zimmern Wang

Boston Medical Center, 4th Floor, FGH Building, 820 Harrison Avenue, Boston, Massachusetts 02118

Ramachandra Dasari, Michael S. Feld

Massachusetts Institute of Technology, G.R. Harrison Spectroscopy Laboratory, Building 6-205, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139

Gregory Grillone

Boston Medical Center, 4th Floor, FGH Building, 820 Harrison Avenue, Boston, Massachusetts 02118

J. Biomed. Opt. 13(6), 064034 (December 10, 2008). doi:10.1117/1.2992139
History: Received April 02, 2008; Revised July 16, 2008; Accepted July 23, 2008; Published December 10, 2008
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In order to evaluate the impact of anatomy on the spectral properties of oral tissue, we used reflectance and fluorescence spectroscopy to characterize nine different anatomic sites. All spectra were collected in vivo from healthy oral mucosa. We analyzed 710 spectra collected from the oral cavity of 79 healthy volunteers. From the spectra, we extracted spectral parameters related to the morphological and biochemical properties of the tissue. The parameter distributions for the nine sites were compared, and we also related the parameters to the physical properties of the tissue site. k-Means cluster analysis was performed to identify sites or groups of sites that showed similar or distinct spectral properties. For the majority of the spectral parameters, certain sites or groups of sites exhibited distinct parameter distributions. Sites that are normally keratinized, most notably the hard palate and gingiva, were distinct from nonkeratinized sites for a number of parameters and frequently clustered together. The considerable degree of spectral contrast (differences in the spectral properties) between anatomic sites was also demonstrated by successfully discriminating between several pairs of sites using only two spectral parameters. We tested whether the 95% confidence interval for the distribution for each parameter, extracted from a subset of the tissue data could correctly characterize a second set of validation data. Excellent classification accuracy was demonstrated. Our results reveal that intrinsic differences in the anatomy of the oral cavity produce significant spectral contrasts between various sites, as reflected in the extracted spectral parameters. This work provides an important foundation for guiding the development of spectroscopic-based diagnostic algorithms for oral cancer.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Sasha McGee ; Jelena Mirkovic ; Vartan Mardirossian ; Alphi Elackattu ; Chung-Chieh Yu, et al.
"Model-based spectroscopic analysis of the oral cavity: impact of anatomy", J. Biomed. Opt. 13(6), 064034 (December 10, 2008). ; http://dx.doi.org/10.1117/1.2992139


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