Research Papers

Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats

[+] Author Affiliations
Seonkyung Lee, Danthu H. Vu, Michael F. Hinds, Steven J. Davis

Physical Sciences Inc., 20 New England Business Center, Andover, Massachusetts 01810-1077

Alvin Liang, Tayyaba Hasan

Massachusetts General Hospital, Wellman Laboratories of Photomedicine, 40 Blossom Street, Boston, Massachusetts 02114-2605

J. Biomed. Opt. 13(6), 064035 (December 18, 2008). doi:10.1117/1.3042265
History: Received July 11, 2008; Revised October 24, 2008; Accepted October 25, 2008; Published December 18, 2008; December 29, 2008
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Photodynamic therapy (PDT) is a promising cancer treatment that involves optical excitation of photosensitizers that promote oxygen molecules to the metastable O2(a1Δ) state (singlet oxygen). This species is believed to be responsible for the destruction of cancerous cells during PDT. We describe a fiber optic-coupled, pulsed diode laser-based diagnostic for singlet oxygen. We use both temporal and spectral filtering to enhance the detection of the weak O2(aX) emission near 1.27μm. We present data that demonstrate real-time singlet oxygen production in tumor-laden rats with chlorin e6 and 5-aminolevulinic acid-induced protoporphyrin photosensitizers. We also observe a positive correlation between post-PDT treatment regression of the tumors and the relative amount of singlet oxygen measured. These results are promising for the development of the sensor as a real-time dosimeter for PDT.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Topics

Oxygen ; Lasers

Citation

Seonkyung Lee ; Danthu H. Vu ; Michael F. Hinds ; Steven J. Davis ; Alvin Liang, et al.
"Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats", J. Biomed. Opt. 13(6), 064035 (December 18, 2008). ; http://dx.doi.org/10.1117/1.3042265


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