Research Papers

Bioimaging assessment and effect of skin wound healing using bone-marrow-derived mesenchymal stromal cells with the artificial dermis in diabetic rats

[+] Author Affiliations
Hirokazu Inoue

Jichi Medical University, Division of Organ Replacement Research, Center for Molecular Medicine and Department of Orthopedics, Tochigi 329-0498, Japan

Takashi Murakami

Jichi Medical University, Division of Organ Replacement Research, Center for Molecular Medicine, Tochigi 329-0498, Japan

Takashi Ajiki

Jichi Medical University, Division of Organ Replacement Research, Center for Molecular Medicine and Department of Orthopedics, Tochigi 329-0498, Japan

Mayumi Hara

Jichi Medical University, Division of Organ Replacement Research, Center for Molecular Medicine, Tochigi 329-0498, Japan

Yuichi Hoshino

Jichi Medical University, Department of Orthopedics, Tochigi 329-0498, Japan

Eiji Kobayashi

Jichi Medical University, Division of Organ Replacement Research, Center for Molecular Medicine, Tochigi 329-0498, Japan

J. Biomed. Opt. 13(6), 064036 (December 18, 2008). doi:10.1117/1.3042266
History: Received April 20, 2008; Revised September 14, 2008; Accepted October 28, 2008; Published December 18, 2008
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We investigate the relationship between the fate and healing effect of transplanted mesenchymal stromal cells (MSCs) in a rat diabetic skin wound model. Rats are treated with streptozotocin to induce diabetic conditions. A full-thickness skin defect is surgically made on the head of diabetic rats, and covered with an artificial dermis impregnated with either bone marrow cells (BMCs) or bone-marrow-derived MSCs from firefly luciferase (luc) transgenic (Tg) rats. Wound healing is evaluated using planimetry and immunohistochemistry, and the fate of transplanted MSCs is determined using in-vivo luminescent imaging. The diabetic wound treated with MSCs-impregnated artificial dermis is significantly smaller than that treated with artificial dermis alone at 1week postoperation. Photons of luc+ MSCs are detected at the transplanted site during healing (3weeks), whereas those of luc+ MSCs are depleted only after 1week postimplantation. Immunohistochemistry at the healing site treated with MSCs demonstrates that CD31+ vessels increase with expression of vascular endothelial growth factor, suggesting that MSCs accelerate angiogenesis. These findings suggest that transplanted MSCs could be retained at wound sites during the healing process in a diabetic rat model, and subsequently promote wound healing through angiogenesis.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Topics

Bone ; Skin ; Wound healing

Citation

Hirokazu Inoue ; Takashi Murakami ; Takashi Ajiki ; Mayumi Hara ; Yuichi Hoshino, et al.
"Bioimaging assessment and effect of skin wound healing using bone-marrow-derived mesenchymal stromal cells with the artificial dermis in diabetic rats", J. Biomed. Opt. 13(6), 064036 (December 18, 2008). ; http://dx.doi.org/10.1117/1.3042266


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