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Research Papers

Two-photon imaging using adaptive phase compensated ultrashort laser pulses

[+] Author Affiliations
Peng Xi

Shanghai Jiao Tong University, Department of Biomedical Engineering, Institute for Laser Medicine and Biophotonics, Shanghai 200240 China

Yair Andegeko, Dmitry Pestov, Vadim V. Lovozoy, Marcos Dantus

Michigan State University, Department of Chemistry, East Lansing, Michigan 48824

J. Biomed. Opt. 14(1), 014002 (January 26, 2009). doi:10.1117/1.3059629
History: Received April 23, 2008; Revised November 10, 2008; Accepted November 10, 2008; Published January 26, 2009
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An adaptive pulse shaper controlled by multiphoton intrapulse interference phase scanning (MIIPS) was used, together with a prism-pair, to measure and cancel high-order phase distortions introduced by a high-numerical-aperture objective and other dispersive elements of a two-photon laser-scanning microscope. The delivery of broad-bandwidth (100nm), sub-12-fs pulses was confirmed by interferometric autocorrelation measurements at the focal plane. A comparison of two-photon imaging with transform-limited and second-order-dispersion compensated laser pulses of the same energy showed a 6-to-11-fold improvement in the two-photon excitation fluorescence signal when applied to cells and tissue, and up to a 19-fold improvement in the second harmonic generation signal from a rat tendon specimen.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Peng Xi ; Yair Andegeko ; Dmitry Pestov ; Vadim V. Lovozoy and Marcos Dantus
"Two-photon imaging using adaptive phase compensated ultrashort laser pulses", J. Biomed. Opt. 14(1), 014002 (January 26, 2009). ; http://dx.doi.org/10.1117/1.3059629


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