Research Papers

Quantitative imaging of scattering changes associated with epithelial proliferation, necrosis, and fibrosis in tumors using microsampling reflectance spectroscopy

[+] Author Affiliations
Venkataramanan Krishnaswamy

Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, New Hampshire 03755

P. Jack Hoopes

Dartmouth Medical School, Departments of Surgery and Medicine, Borwell Research Building, Lebanon, New Hampshire 03756 and Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, New Hampshire 03755

Kimberley S. Samkoe

Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, New Hampshire 03755

Julia A. O’Hara

Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, New Hampshire 03755 and Dartmouth Medical School, Department of Radiology, Borwell Research Building, Lebanon, New Hampshire 03756

Tayyaba Hasan

Massachusetts General Hospital, Wellman Center for Photomedicine, 40 Blossom Street, Boston, Massachusetts 02114 and Harvard Medical School, Department of Dermatology, 55 Fruit Street, Boston, Massachusetts 02115

Brian W. Pogue

Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, New Hampshire 03755 and Dartmouth Medical School, Department of Surgery, Borwell Research Building, Lebanon, New Hampshire 03756 and Massachusetts General Hospital, Wellman Center for Photomedicine, 40 Blossom Street, Boston, Massachusetts 02114

J. Biomed. Opt. 14(1), 014004 (February 12, 2009). doi:10.1117/1.3065540
History: Received April 07, 2008; Revised November 09, 2008; Accepted November 13, 2008; Published February 12, 2009
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Highly localized reflectance measurements can be used to directly quantify scatter changes in tissues. We present a microsampling approach that is used to raster scan tumors to extract parameters believed to be related to the tissue ultrastructure. A confocal reflectance imager was developed to examine scatter changes across pathologically distinct regions within tumor tissues. Tissue sections from two murine tumors, AsPC-1 pancreas tumor and the Mat-LyLu Dunning prostate tumor, were imaged. After imaging, histopathology-guided region-of-interest studies of the images allowed analysis of the variations in scattering resulting from differences in tissue ultra-structure. On average, the median scatter power of tumor cells with high proliferation index (HPI) was about 26% less compared to tumor cells with low proliferation index (LPI). Necrosis exhibited the lowest scatter power signature across all the tissue types considered, with about 55% lower median scatter power than LPI tumor cells. Additionally, the level and maturity of the tumor’s fibroplastic response was found to influence the scatter signal. This approach to scatter visualization of tissue ultrastructure in situ could provide a unique tool for guiding surgical resection, but this kind of interpretation into what the signal means relative to the pathology is required before proceeding to clinical studies.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Venkataramanan Krishnaswamy ; P. Jack Hoopes ; Kimberley S. Samkoe ; Julia A. O’Hara ; Tayyaba Hasan, et al.
"Quantitative imaging of scattering changes associated with epithelial proliferation, necrosis, and fibrosis in tumors using microsampling reflectance spectroscopy", J. Biomed. Opt. 14(1), 014004 (February 12, 2009). ; http://dx.doi.org/10.1117/1.3065540


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