Research Papers

Noninvasive in vitro and in vivo assessment of epidermal hyperkeratosis and dermal fibrosis in atopic dermatitis

[+] Author Affiliations
Jyh-Hong Lee

National Taiwan University Hospital, and National Taiwan University College of Medicine, Department of Pediatrics, Taipei, 100, Taiwan

Szu-Yu Chen, Che-Hang Yu

National Taiwan University, Graduate Institute of Photonics and Optoelectronics, and Department of Electrical Engineering, Taipei, 106, Taiwan

Shih-Wei Chu

Ten-Chan General Hospital, Department of Dermatology, Chung-Li, 320, Tao-Yuan County, Taiwan

Li-Fang Wang

National Taiwan University Hospital, and National Taiwan University College of Medicine, Department of Dermatology, Taipei, 100, Taiwan

Chi-Kuang Sun

National Taiwan University, Graduate Institute of Photonics and Optoelectronics, and Department of Electrical Engineering, Taipei, 106, Taiwan and Academia Sinica, Research Center for Applied Sciences, Taipei, 115, Taiwan

Bor-Luen Chiang

National Taiwan University Hospital, Department of Pediatrics, and National Taiwan University College of Medicine, Graduate Institute of Clinical Medicine, Taipei, 100, Taiwan

J. Biomed. Opt. 14(1), 014008 (February 11, 2009). doi:10.1117/1.3077182
History: Received May 06, 2008; Revised November 10, 2008; Accepted November 18, 2008; Published February 11, 2009
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Atopic dermatitis (AD) is characterized by hyperkeratosis of epidermis and fibrosis within dermis in chronic skin lesions. Thus far, the histology of skin lesions has been evaluated only by examination of excised specimens. A noninvasive in vivo tool is essential to evaluate the histopathological changes during the clinical course of AD. We used Cr:forsterite laser-based multimodality nonlinear microscopy to analyze the endogenous molecular signals, including third-harmonic generation (THG), second-harmonic generation (SHG), and two-photon fluorescence (TPF) from skin lesions in AD. Significant differences in thickness of epidermis and stratum corneum (SC), and modified degrees of fibrosis in dermis (measured by THG signals and SHG signals, respectively), are clearly demonstrated in in vitro studies. Increased TPF levels are positively associated with the levels of the THG signals from the SC. Our in vitro observations of histological changes are replicated in the in vivo studies. These findings were reproducible in skin lesions from human AD. For the first time, we demonstrate the feasibility of preclinical applications of Cr:forsterite laser-based nonlinear microscopy. Our findings suggest that the optical signatures of THG, TPF, and SHG can be used as molecular markers to assess the pathophysiological process of AD and the effects of local treatment.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Jyh-Hong Lee ; Szu-Yu Chen ; Che-Hang Yu ; Shih-Wei Chu ; Li-Fang Wang, et al.
"Noninvasive in vitro and in vivo assessment of epidermal hyperkeratosis and dermal fibrosis in atopic dermatitis", J. Biomed. Opt. 14(1), 014008 (February 11, 2009). ; http://dx.doi.org/10.1117/1.3077182


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