Research Papers

Proof-of-principle demonstration of a Mueller matrix decomposition method for polarized light tissue characterization in vivo

[+] Author Affiliations
Michael F. G. Wood, Nirmalya Ghosh, Eduardo H. Moriyama, Brian C. Wilson

Ontario Cancer Institute, Division of Biophysics and Bioimaging, University Health Network, 610 University Avenue, Toronto, Ontario M5G 2M9 Canada and University of Toronto, Department of Medical Biophysics, 610 University Avenue, Toronto, Ontario M5G 2M9 Canada

I. Alex Vitkin

Ontario Cancer Institute, Division of Biophysics and Bioimaging, University Health Network, 610 University Avenue, Toronto, Ontario M5G 2M9 Canada and University of Toronto, Department of Medical Biophysics and Radiation Oncology, 610 University Avenue, Toronto, Ontario M5G 2M9 Canada

J. Biomed. Opt. 14(1), 014029 (February 04, 2009). doi:10.1117/1.3065545
History: Received August 15, 2008; Revised November 19, 2008; Accepted November 19, 2008; Published February 04, 2009
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We demonstrate the first in vivo use of a Mueller matrix decomposition method for polarization-based characterization of tissue. Collagenase is injected into a region of dermal tissue in a dorsal skin window chamber in a nude mouse to alter the structure of the extracellular matrix. Mueller matrices for polarized light transmitted through the window chamber in the collagenase-treated region, as well as a distal control region, are measured. From the measured matrices, the individual constituent polarization properties of the tissue are extracted through polar matrix decomposition. Large decreases in birefringence and depolarization are seen in the collagenase-treated region due to the destruction of collagen, showing the potential for this method to monitor the organization and structural anisotropy of tissue. This study represents the first in vivo demonstration of a Mueller matrix decomposition method for polarimetric tissue characterization.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Michael F. G. Wood ; Nirmalya Ghosh ; Eduardo H. Moriyama ; Brian C. Wilson and I. Alex Vitkin
"Proof-of-principle demonstration of a Mueller matrix decomposition method for polarized light tissue characterization in vivo", J. Biomed. Opt. 14(1), 014029 (February 04, 2009). ; http://dx.doi.org/10.1117/1.3065545


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