Special Section on Nanophotonics for Diagnostics, Protection, and Treatment of Cancer and Inflammatory Diseases

Fluorescent immunolabeling of cancer cells by quantum dots and antibody scFv fragment

[+] Author Affiliations
Tatiana A. Zdobnova

Russian Academy of Sciences, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya, 16/10, 117997 GSP, Moscow V-437, Russia and Russian Academy of Sciences, Institute of Applied Physics, 46 Ul’yanov Street, Nizhny Novgorod 603950, Russia

Sergey G. Dorofeev, Piter N. Tananaev, Roman B. Vasiliev

Lomonosov Moscow State University, GSP-2, Leninskie Gory, Moscow, 119992, Russia

Taras G. Balandin, Eveline F. Edelweiss, Oleg A. Stremovskiy

Russian Academy of Sciences, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya, 16/10, 117997 GSP, Moscow V-437, Russia

Irina V. Balalaeva, Ilya V. Turchin

Russian Academy of Sciences, Institute of Applied Physics, 46 Ul’yanov Street, Nizhny Novgorod 603950, Russia

Ekaterina N. Lebedenko

Russian Academy of Sciences, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya, 16/10, 117997 GSP, Moscow V-437, Russia

Vladimir P. Zlomanov

Lomonosov Moscow State University, GSP-2, Leninskie Gory, Moscow, 119992, Russia

Sergey M. Deyev

Russian Academy of Sciences, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya, 16/10, 117997 GSP, Moscow V-437, Russia

J. Biomed. Opt. 14(2), 021004 (April 23, 2009). doi:10.1117/1.3122775
History: Received July 08, 2008; Revised March 04, 2009; Accepted March 05, 2009; Published April 23, 2009
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Semiconductor quantum dots (QDs) coupled with cancer-specific targeting ligands are new promising agents for fluorescent visualization of cancer cells. Human epidermal growth factor receptor 2/neu (HER2/neu), overexpressed on the surface of many cancer cells, is an important target for cancer diagnostics. Antibody scFv fragments as a targeting agent for direct delivery of fluorophores offer significant advantages over full-size antibodies due to their small size, lower cross-reactivity, and immunogenicity. We have used quantum dots linked to anti-HER2/neu 4D5 scFv antibody to label HER2/neu-overexpressing live cells. Labeling of target cells was shown to have high brightness, photostability, and specificity. The results indicate that construction based on quantum dots and scFv antibody can be successfully used for cancer cell visualization.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Tatiana A. Zdobnova ; Sergey G. Dorofeev ; Piter N. Tananaev ; Roman B. Vasiliev ; Taras G. Balandin, et al.
"Fluorescent immunolabeling of cancer cells by quantum dots and antibody scFv fragment", J. Biomed. Opt. 14(2), 021004 (April 23, 2009). ; http://dx.doi.org/10.1117/1.3122775


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