Research Papers

Effects of Er:YAG laser irradiation and manipulation treatments on dentin components, part 2: energy-dispersive X-ray fluorescence spectrometry study

[+] Author Affiliations
Luís Eduardo Silva Soares

Vale do Paraíba University, UNIVAP, Dental Materials and Operative Dentistry Department, School of Dentistry Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB, Av. Shishima Hifumi, 2911, 12244-000 São José dos Campos, São Paulo, Brazil

Ana Maria do Espírito Santo

Vale do Paraíba University, UNIVAP, Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB, Av. Shishima Hifumi, 2911, 12244-000 São José dos Campos, São Paulo, Brazil

Aldo Brugnera, Fátima Antônia Aparecida Zanin

Vale do Paraíba University, UNIVAP, Research and Development Institute, IP&D, Dental Laser Center, Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, São Paulo, Brazil

Airton Abrahão Martin

Vale do Paraíba University, UNIVAP, Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB, Av. Shishima Hifumi, 2911, 12244-000 São José dos Campos, São Paulo, Brazil

J. Biomed. Opt. 14(2), 024002 (March 31, 2009). doi:10.1117/1.3103287
History: Received May 20, 2008; Revised December 10, 2008; Accepted December 22, 2008; Published March 31, 2009
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The effects of laser etching, decontamination, and storage treatments on dentin components were studied by energy-dispersive X-ray fluorescence spectrometry (EDXRF). Thirty bovine incisors were prepared to expose the dentin surface and then divided into two main groups based upon the decontamination process and storage procedure: autoclaved (group A, n=15) or stored in aqueous thymol solution (group B, n=15). The surfaces of the dentin slices were schematically divided into four areas, with each one corresponding to a treatment subgroup. The specimens were either etched with phosphoric acid (control subgroup) or irradiated with erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (subgroups: I-80mJ, II-120mJ, and III-180mJ). Samples were analyzed by micro-EDXRF, yielding three spectra for each area (before and after treatment). Surface mappings covering an area of 80×60 points with steps of 20μm were also performed on selected specimens. The amount of Ca and P in group A specimens decreased significantly (P<0.05) after the acid etching and the CaP ratio increased (P<0.001). Er:YAG laser-etching using lower laser energies did not produce significant changes in dentin components. The mapping data support the hypothesis that acid etching on dentin produced a more chemically homogeneous surface and thus a more favorable surface for the diffusion of adhesive monomers.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Luís Eduardo Silva Soares ; Ana Maria do Espírito Santo ; Aldo Brugnera, Junior ; Fátima Antônia Aparecida Zanin and Airton Abrahão Martin
"Effects of Er:YAG laser irradiation and manipulation treatments on dentin components, part 2: energy-dispersive X-ray fluorescence spectrometry study", J. Biomed. Opt. 14(2), 024002 (March 31, 2009). ; http://dx.doi.org/10.1117/1.3103287


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