Research Papers

Quantitation and mapping of tissue optical properties using modulated imaging

[+] Author Affiliations
David J. Cuccia

Modulated Imaging, Inc., 1002 Health Sciences Road, Irvine, California 92612

Frederic Bevilacqua, Anthony J. Durkin, Frederick R. Ayers, Bruce J. Tromberg

University of California, Irvine, Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92612

J. Biomed. Opt. 14(2), 024012 (April 03, 2009). doi:10.1117/1.3088140
History: Received April 03, 2006; Revised December 31, 2008; Accepted January 14, 2009; Published April 03, 2009
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We describe the development of a rapid, noncontact imaging method, modulated imaging (MI), for quantitative, wide-field characterization of optical absorption and scattering properties of turbid media. MI utilizes principles of frequency-domain sampling and model-based analysis of the spatial modulation transfer function (s-MTF). We present and compare analytic diffusion and probabilistic Monte Carlo models of diffuse reflectance in the spatial frequency domain. Next, we perform MI measurements on tissue-simulating phantoms exhibiting a wide range of l* values (0.5mmto3mm) and (μsμa) ratios (8 to 500), reporting an overall accuracy of approximately 6% and 3% in absorption and reduced scattering parameters, respectively. Sampling of only two spatial frequencies, achieved with only three camera images, is found to be sufficient for accurate determination of the optical properties. We then perform MI measurements in an in vivo tissue system, demonstrating spatial mapping of the absorption and scattering optical contrast in a human forearm and dynamic measurements of a forearm during venous occlusion. Last, metrics of spatial resolution are assessed through both simulations and measurements of spatially heterogeneous phantoms.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

David J. Cuccia ; Frederic Bevilacqua ; Anthony J. Durkin ; Frederick R. Ayers and Bruce J. Tromberg
"Quantitation and mapping of tissue optical properties using modulated imaging", J. Biomed. Opt. 14(2), 024012 (April 03, 2009). ; http://dx.doi.org/10.1117/1.3088140


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