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Research Papers

In vivo confocal imaging of epidermal cell migration and dermal changes post nonablative fractional resurfacing: study of the wound healing process with corroborated histopathologic evidence

[+] Author Affiliations
Oliver F. Stumpp, Vikramaditya P. Bedi, Danica Wyatt, Diana Lac

Solta Medical, 25881 Industrial Boulevard, Hayward, California 94545

Zakia Rahman

Solta Medical, 25881 Industrial Boulevard, Hayward, California 94545 and Stanford University, School of Medicine, Stanford, California 94305

Kin F. Chan

Solta Medical, 25881 Industrial Boulevard, Hayward, California 94545

J. Biomed. Opt. 14(2), 024018 (October 29, 2008January 16, 2009January 20, 2009April 23, 2009). doi:10.1117/1.3103316
History: Received October 29, 2008; Revised January 16, 2009; Accepted January 20, 2009; Published April 23, 2009
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In vivo wound healing response post nonablative fractional laser treatment is evaluated. Seven healthy subjects receive treatments with a Fraxel re:store™ laser system on the forearm with pulse energies ranging from 10to70mJ. The treatment sites are imaged at 1-h increments up to 40h using confocal microscope z-stacks using 10-μm-depth spacing. At least five individual microscopic treatment zones are imaged per subject, time point, and treatment energy. Images are analyzed for tissue structure and morphology to classify each lesion as healed or not healed, depending on epidermal re-epithelialization at each time point and treatment energy. Probit analysis is used to statistically determine the ED50 and ED84 probabilities for a positive dose response (healed lesion) as a function of treatment energy. Confocal observations reveal epidermal keratinocyte migration patterns confirmed with histological analysis using hematoxylin and eosin (HE) and lactate dehydrogenase (LDH) staining at 10mJ at 0, 7, 16, and 24-h post-treatment. Results indicate that more time is required to conclude re-epithelialization with larger lesion sizes (all less than 500μm) corresponding to higher treatment energies. For the entire pulse energy range tested, epidermal re-epithelialization concludes between 10to22-h post-treatment for ED50 and 13to28h for ED84.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Oliver F. Stumpp ; Zakia Rahman ; Kin F. Chan ; Vikramaditya P. Bedi ; Danica Wyatt, et al.
"In vivo confocal imaging of epidermal cell migration and dermal changes post nonablative fractional resurfacing: study of the wound healing process with corroborated histopathologic evidence", J. Biomed. Opt. 14(2), 024018 (October 29, 2008January 16, 2009January 20, 2009April 23, 2009). ; http://dx.doi.org/10.1117/1.3103316


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