Research Papers

Re-evaluation of model-based light-scattering spectroscopy for tissue spectroscopy

[+] Author Affiliations
Condon Lau, Obrad Šćepanović, Jelena Mirkovic, Sasha McGee, Chung-Chieh Yu

Massachusetts Institute of Technology, George R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139

Stephen Fulghum

Newton Laboratories, 23 Cummings Park, Woburn, Massachusetts 01801

Michael Wallace

Mayo Clinic, Department of Gastroenterology and Hepatology, 4500 San Pablo Road, Jacksonville, Florida 32224

James Tunnell

University of Texas at Austin, Department of Biomedical Engineering, 1 University Station, C0800 Austin, Texas 78712-0238

Kate Bechtel, Michael Feld

Massachusetts Institute of Technology, George R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139

J. Biomed. Opt. 14(2), 024031 (April 14, 2009). doi:10.1117/1.3116708
History: Received April 17, 2008; Revised February 09, 2009; Accepted February 10, 2009; Published April 14, 2009
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Model-based light scattering spectroscopy (LSS) seemed a promising technique for in-vivo diagnosis of dysplasia in multiple organs. In the studies, the residual spectrum, the difference between the observed and modeled diffuse reflectance spectra, was attributed to single elastic light scattering from epithelial nuclei, and diagnostic information due to nuclear changes was extracted from it. We show that this picture is incorrect. The actual single scattering signal arising from epithelial nuclei is much smaller than the previously computed residual spectrum, and does not have the wavelength dependence characteristic of Mie scattering. Rather, the residual spectrum largely arises from assuming a uniform hemoglobin distribution. In fact, hemoglobin is packaged in blood vessels, which alters the reflectance. When we include vessel packaging, which accounts for an inhomogeneous hemoglobin distribution, in the diffuse reflectance model, the reflectance is modeled more accurately, greatly reducing the amplitude of the residual spectrum. These findings are verified via numerical estimates based on light propagation and Mie theory, tissue phantom experiments, and analysis of published data measured from Barrett’s esophagus. In future studies, vessel packaging should be included in the model of diffuse reflectance and use of model-based LSS should be discontinued.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Condon Lau ; Obrad Šćepanović ; Jelena Mirkovic ; Sasha McGee ; Chung-Chieh Yu, et al.
"Re-evaluation of model-based light-scattering spectroscopy for tissue spectroscopy", J. Biomed. Opt. 14(2), 024031 (April 14, 2009). ; http://dx.doi.org/10.1117/1.3116708


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