Research Papers

Influence of oxygen saturation on the optical scattering properties of human red blood cells in the spectral range 250to2000nm

[+] Author Affiliations
Moritz Friebel, Jürgen Helfmann

Laser- und Medizin-Technologie GmbH, Berlin, Fabeckstrasse 60-62, 14195 Berlin, Germany

Uwe Netz

Charité-Universitätsmedizin Berlin, Medizinische Physik und Optische Diagnostik, Fabeckstrasse 60-62, 14195 Berlin, Germany

Martina Meinke

Charité-Universitätsmedizin Berlin, Department of Dermatology and Allergology, Charitéplatz 1, 10117 Berlin, Germany

J. Biomed. Opt. 14(3), 034001 (May 11, 2009). doi:10.1117/1.3127200
History: Received September 05, 2008; Revised February 12, 2009; Accepted March 09, 2009; Published May 11, 2009
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The intrinsic optical parameters absorption coefficient μa, scattering coefficient μs, anisotropy factor g, and effective scattering coefficient μs were determined for human red blood cell (RBC) suspensions of hematocrit 33.2% dependent on the oxygen saturation (SAT O2) in the wavelength range 250to2000nm, including the range above 1100nm, about which there are no data available in the literature. Integrating sphere measurements of light transmittance and reflectance in combination with inverse Monte Carlo simulation were carried out for SAT O2 levels of 100 and 0%. In the wavelength range up to 1200nm, the absorption behavior is determined by the hemoglobin absorption. The spectral range above the cells’ absorption shows no dependence on SAT O2 and approximates the absorption of water with values 20 to 30% below the respective values for water. Parameters μs and g are significantly influenced by the SAT O2-induced absorption changes. Above 600nm, μs decreases continuously from values of 85mm1 to values of 30mm1 at 2000nm. The anisotropy factor shows a slight decrease with wavelengths above 600nm. In the spectral regions of 1450 and 1900nm where water has local absorption maxima, g shows a significant decrease down to 0.85, whereas μs increases.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Moritz Friebel ; Jürgen Helfmann ; Uwe Netz and Martina Meinke
"Influence of oxygen saturation on the optical scattering properties of human red blood cells in the spectral range 250to2000nm", J. Biomed. Opt. 14(3), 034001 (May 11, 2009). ; http://dx.doi.org/10.1117/1.3127200


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