Research Papers

Effect of low-level laser treatment of tissue-engineered skin substitutes: contraction of collagen lattices

[+] Author Affiliations
Gideon Ho

Exploit Technologies, Biomedical Sciences Division, Agency of Science and Technology (A*STAR), 30 Biopolis Street, #09-02 Matrix, Singapore 138671, Singapore

Joseph Barbenel

University of Strathclyde, Royal College, Department of Electronics and Electrical Engineering, 204 George Street, Glasgow, Scotland G1 1XQ, United Kingdom

M. Helen Grant

University of Strathclyde, Bioengineering Unit, Wolfson Centre, Glasgow, Scotland G4 0NW, United Kingdom

J. Biomed. Opt. 14(3), 034002 (May 12, 2009). doi:10.1117/1.3127201
History: Received October 29, 2008; Revised March 04, 2009; Accepted March 10, 2009; Published May 12, 2009
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Fibroblast-populated collagen lattices (FPCL) are widely used in tissue-engineered artificial skin substitutes, but their main drawback is that interaction of fibroblasts and matrix causes contraction of the lattice, reducing it to about 20% of its original area. The effect of low-level laser treatment (LLLT) on the behavior of 3T3 fibroblasts seeded in collagen lattices containing 20% chondroitin-6-sulphate was investigated to determine whether LLLT could control the contraction of FPCL. A He-Ne laser was used at 632.8nm to deliver a 5-mW continuous wave with fluences from 1to4Jcm2. Laser treatment at 3Jcm2 increased contraction of collagen lattices in the absence of cells but decreased contraction of cell seeded lattices over a 7-day period. The effect was energy dependent and was not observed at 1, 2, or 4Jcm2. There was no alteration in fibroblast viability, morphology, or mitochondrial membrane potential after any laser treatments, but the distribution of actin fibers within the cells and collagen fibers in the matrices was disturbed at 3Jcm2. These effects contribute to the decrease in contraction observed. LLLT may offer a means to control contraction of FPCL used as artificial skin substitutes.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Gideon Ho ; Joseph Barbenel and M. Helen Grant
"Effect of low-level laser treatment of tissue-engineered skin substitutes: contraction of collagen lattices", J. Biomed. Opt. 14(3), 034002 (May 12, 2009). ; http://dx.doi.org/10.1117/1.3127201


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