Research Papers

Sensitivity and specificity for detecting basal cell carcinomas in Mohs excisions with confocal fluorescence mosaicing microscopy

[+] Author Affiliations
Daniel S. Gareau

Memorial Sloan-Kettering Cancer Center, Dermatology Service, Room 230, 160 East 53rd Street, New York, New York 10022

Julie K. Karen, Stephen W. Dusza, Marie Tudisco, Kishwer S. Nehal, Milind Rajadhyaksha

Memorial Sloan-Kettering Cancer Center, Dermatology Service, 60 East 53rd Street, New York, New York 10022

J. Biomed. Opt. 14(3), 034012 (June 24, 2009). doi:10.1117/1.3130331
History: Received August 13, 2008; Revised February 13, 2009; Accepted March 19, 2009; Published June 24, 2009
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Recent studies have demonstrated the ability of confocal fluorescence mosaicing microscopy to rapidly detect basal cell carcinomas (BCCs) directly in thick and fresh Mohs surgical excisions. Mosaics of confocal images display large areas of tissue with high resolution and magnification equivalent to 2×, which is the standard magnification when examining pathology. Comparison of mosaics to Mohs frozen histopathology was shown to be excellent for all types of BCCs. However, comparisons in the previous studies were visual and qualitative. In this work, we report the results of a semiquantitative preclinical study in which 45 confocal mosaics are blindly evaluated for the presence (or absence) of BCC tumor. The evaluations are made by two clinicians: a senior Mohs surgeon with prior expertise in interpreting confocal images, and a novice Mohs fellow with limited experience. The blinded evaluation is compared to the gold standard of frozen histopathology. BCCs are detected with an overall sensitivity of 96.6%, specificity of 89.2%, positive predictive value of 93.0%, and negative predictive value of 94.7%. The results demonstrate the potential clinical utility of confocal mosaicing microscopy toward rapid surgical pathology at the bedside to expedite and guide surgery.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Daniel S. Gareau ; Julie K. Karen ; Stephen W. Dusza ; Marie Tudisco ; Kishwer S. Nehal, et al.
"Sensitivity and specificity for detecting basal cell carcinomas in Mohs excisions with confocal fluorescence mosaicing microscopy", J. Biomed. Opt. 14(3), 034012 (June 24, 2009). ; http://dx.doi.org/10.1117/1.3130331


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