Research Papers

Morphological studies of living cells using gold nanoparticles and dark-field optical section microscopy

[+] Author Affiliations
Chia-Wei Lee

Academia Sinica, Research Center for Applied Sciences, No. 128 Academic Road Section 2, Taipei, 11529, Taiwan and National Taiwan University, Department of Material Science and Engineering, No. 1 Roosevelt Road Section 4, Taipei, 10617, Taiwan

Miin Jang Chen

National Taiwan University, Department of Material Science and Engineering, No. 1 Roosevelt Road Section 4, Taipei, 10617, Taiwan

Ji-Yen Cheng

Academia Sinica, Research Center for Applied Sciences, No. 128 Academic Road Section 2, Taipei, 11529, Taiwan

Pei-Kuen Wei

Academia Sinica, Research Center for Applied Sciences, No. 128 Academic Road Section 2, Taipei, 11529, Taiwan and National Taiwan Ocean University, Keelung Department of Optoelectronics, No. 2 Beining Road, Jhongjheng District, Keelung City, 202, Taiwan and National Yang-Ming University, Institute of Biophotonics, 115 Li-Non Street Section 2, Taipei, 112, Taiwan

J. Biomed. Opt. 14(3), 034016 (June 04, 2009). doi:10.1117/1.3147390
History: Received October 07, 2008; Revised March 12, 2009; Accepted March 25, 2009; Published June 04, 2009
Text Size: A A A

The morphologic changes of living cells under drug interactions were studied by using 80-nm gold nanoparticles and dark-field optical section microscopy. The gold nanoparticles were coated with poly (L-lysine), which attached to the membranes of various cells by way of electrostatic attractive force. A three-dimensional (3-D) morphological image was obtained by measuring the peak scattering intensities of gold nanoparticles at different focal planes. An algorithm for the reconstruction of 3-D cell morphology was presented. With the measured nanoparticle images and calculations, we show morphologic changes of lung cancer cells under the interaction of cytochalasin D drug at different times.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Chia-Wei Lee ; Miin Jang Chen ; Ji-Yen Cheng and Pei-Kuen Wei
"Morphological studies of living cells using gold nanoparticles and dark-field optical section microscopy", J. Biomed. Opt. 14(3), 034016 (June 04, 2009). ; http://dx.doi.org/10.1117/1.3147390


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.