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Research Papers

Dual-spectrum laser source based on fiber continuum generation for integrated optical coherence and multiphoton microscopy

[+] Author Affiliations
Benedikt W. Graf, Zhi Jiang, Haohua Tu, Stephen A. Boppart

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, Department of Electrical and Computer Engineering, Biophotonics Imaging Laboratory, 405 North Mathews Avenue, Urbana, Illinois 61801

J. Biomed. Opt. 14(3), 034019 (November 14, 2008March 27, 2009March 31, 2009June 04, 2009). doi:10.1117/1.3147422
History: Received November 14, 2008; Revised March 27, 2009; Accepted March 31, 2009; Published June 04, 2009
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A single-laser dual-spectrum source designed for integrated optical coherence and multiphoton microscopy is demonstrated. The source implements the laser characteristics needed to optimally perform both modalities while extending the spectral range for this imaging technique. It consists of a widely tunable, mode-locked, Ti-sapphire laser with a portion of its output spectrally broadened via continuum generation in a photonic crystal fiber. The continuum-broadened beam allows for enhanced optical sectioning with optical coherence microscopy, while the unbroadened beam from the ultrashort-pulse Ti-sapphire laser optimally excites fluorescent markers. The noise power of the continuum-broadened beam is less than 1.1dBmHz higher than the Ti-sapphire laser in the range from 1Hzto25MHz, and the fiber shows no sign of damage after 100h of use. We demonstrate the use of this source across a wide spectral range by imaging green fluorescent protein-transfected mouse fibroblast cells costained with fluorescent dyes that are maximally excited at various wavelengths. Images of unstained in vivo human skin are also presented. This source extends the feasibility of this integrated imaging modality and will facilitate new investigations in in vivo microscopy, tissue engineering, and cell biology.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Benedikt W. Graf ; Zhi Jiang ; Haohua Tu and Stephen A. Boppart
"Dual-spectrum laser source based on fiber continuum generation for integrated optical coherence and multiphoton microscopy", J. Biomed. Opt. 14(3), 034019 (November 14, 2008March 27, 2009March 31, 2009June 04, 2009). ; http://dx.doi.org/10.1117/1.3147422


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