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Research Papers

Diffuse reflectance spectroscopy characterization of hemoglobin and intralipid solutions: in vitro measurements with continuous variation of absorption and scattering

[+] Author Affiliations
Sergio E. Hernández

Universidad de La Laguna, Departamento de Física Fundamental y Experimental, Electrónica y Sistemas, Facultad de Física, La Laguna Tenerife, 38206, Spain

Vicente D. Rodríguez, Justo Pérez

Universidad de La Laguna, Departamento de Física Fundamental y Experimental, Electrónica y Sistemas, Facultad de Física, La Laguna Tenerife, 38206, Spain

Felipe A. Martín, Miguel A. Castellano

Universidad de La Laguna, Departamento de Psicobiología y Metodología de las Ciencias del Comportamiento, Facultad de Psicología, La Laguna Tenerife, 38206, Spain

Jose Luis Gonzalez-Mora

Universidad de La Laguna, Departamento de Fisiología, Facultad de Medicina, La Laguna Tenerife, 38206, Spain

J. Biomed. Opt. 14(3), 034026 (June 09, 2009). doi:10.1117/1.3149864
History: Received April 02, 2008; Revised April 03, 2009; Accepted April 08, 2009; Published June 09, 2009
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Absorption and scattering processes in biological tissues are studied through reflectance spectroscopy in tissue-like phantoms. For this aim, an experimental setup is designed to independently control both processes in hemoglobin and intralipid solutions. From the analysis of the obtained spectra, a simple empirical power law equation is found that relates absorbance with scattering and absorption coefficients. This relationship includes three wavelength independent parameters, which can be determined geometry from in vitro measurements for each particular optical optode. The dependence of the optical path length on the absorption and scattering coefficients is also analyzed, and estimations of this parameter for physiological conditions are presented. This study is useful to better understand the scattering phenomena in biological tissue, and to obtain absolute concentration of absorber particles when a homogeneous medium can be assumed.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Sergio E. Hernández ; Vicente D. Rodríguez ; Felipe A. Martín ; Jose Luis Gonzalez-Mora ; Miguel A. Castellano, et al.
"Diffuse reflectance spectroscopy characterization of hemoglobin and intralipid solutions: in vitro measurements with continuous variation of absorption and scattering", J. Biomed. Opt. 14(3), 034026 (June 09, 2009). ; http://dx.doi.org/10.1117/1.3149864


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