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Research Papers

Millimeter-resolution acousto-optic quantitative imaging in a tissue model system

[+] Author Affiliations
Aliaksandr Bratchenia

University of Twente, Institute of Biomedical Technology, Faculty of Science and Technology, P.O. Box 217, 7500 AE Enschede, The Netherlands

Robert Molenaar

University of Twente, Institute of Biomedical Technology, Faculty of Science and Technology, P.O. Box 217, 7500 AE Enschede, The Netherlands

Ton G. van Leeuwen

University of Twente, Institute of Biomedical Technology, Faculty of Science and Technology, P.O Box 217, 7500 AE Enschede, The Netherlands and University of Amsterdam, Academic Medical Center, Biomedical Engineering and Physics, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands

Rob P. H. Kooyman

University of Twente, Institute of Biomedical Technology, Faculty of Science and Technology, P.O. Box 217, 7500 AE Enschede, The Netherlands

J. Biomed. Opt. 14(3), 034031 (December 18, 2008April 16, 2009April 16, 2009June 23, 2009July 09, 2009). doi:10.1117/1.3153894
History: Received December 18, 2008; Revised April 16, 2009; Accepted April 16, 2009; Published June 23, 2009; July 09, 2009
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We have investigated the application of ultrasound modulated coherent light for quantitative determination of the ratio of dye concentrations and total concentration of absorbers in a blood vessel–mimicking sample. A 3-mm-diam tube containing the mixture of dyes inside an Intralipid-based gel with optical properties similar to tissue was interrogated by two different laser wavelengths in combination with intense microsecond ultrasound bursts. The use of calibration curves allowed us to extract quantitative information on the ratio of dye concentrations with the accuracy of better than 15%, as well as on the total concentration. Furthermore, we demonstrated the feasibility to obtain a quantitative 3-D map of the absorbing structure with a spatial resolution of better than 3 mm. These findings give an outlook to apply this technique for noninvasive 3-D mapping of oxygen saturation and total concentration of hemoglobin in tissue.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Aliaksandr Bratchenia ; Robert Molenaar ; Ton G. van Leeuwen and Rob P. H. Kooyman
"Millimeter-resolution acousto-optic quantitative imaging in a tissue model system", J. Biomed. Opt. 14(3), 034031 (December 18, 2008April 16, 2009April 16, 2009June 23, 2009July 09, 2009). ; http://dx.doi.org/10.1117/1.3153894


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