Research Papers

Acoustic fingerprints of dye-labeled protein submicrosphere photoacoustic contrast agents

[+] Author Affiliations
Michael A. McDonald

National Institute of Standards and Technology, Chemical Science and Technology Laboratory, 100 Bureau Drive, Stop 8313, Gaithersburg, Maryland 20899-8313

Ladislav Jankovic, Khalid Shahzad, Michael Burcher

Philips Research USA, 345 Scarborough Road, Briarcliff Manor, New York 10510

King C. P. Li

The Methodist Hospital, Department of Radiology, 6565 Fannin Street D280, Houston, Texas 77030

J. Biomed. Opt. 14(3), 034032 (June 18, 2009). doi:10.1117/1.3155519
History: Received September 29, 2008; Revised March 30, 2009; Accepted April 16, 2009; Published June 18, 2009
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Dye-labeled protein microspheres, submicron in size and capable of producing thermoelastically generated ultrasound in response to laser stimulation, are presented as contrast agents for photoacoustic imaging. Incident laser energy absorbed by fluorescein isothiocyanate (FITC)–labeled elastin submicrospheres results in thermoelastically generated sound production. Plotted A-line graphs reveal a distinctive morphology and a greater than two orders of magnitude increase in signal amplitude subsequent to converting FITC elastin into submicrospheres (despite a four orders of magnitude decrease in concentration). Evidence of nonlinearity and enhancement of ultrasound backscatter indicate a potential use in contrast-enhanced harmonic imaging. Photoacoustic and ultrasound imaging of FITC-elastin submicrospheres in a water-filled phantom vessel shows enhanced contrast at low concentration and clear delineation of the phantom vessel wall.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Michael A. McDonald ; Ladislav Jankovic ; Khalid Shahzad ; Michael Burcher and King C. P. Li
"Acoustic fingerprints of dye-labeled protein submicrosphere photoacoustic contrast agents", J. Biomed. Opt. 14(3), 034032 (June 18, 2009). ; http://dx.doi.org/10.1117/1.3155519


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