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Research Papers

Three-dimensional surface profile intensity correction for spatially modulated imaging

[+] Author Affiliations
Sylvain Gioux

Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215 and Beth Israel Deaconess Medical Center, Department of Medicine, Division of Hematology/Oncology, 330 Brookline Avenue, Boston, Massachusetts 02215

Amaan Mazhar

University of California, Irvine, Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92612

David J. Cuccia

Modulated Imaging Inc., Technology Incubator Office, 1002 Health Sciences Road, Irvine, California 92612

Anthony J. Durkin, Bruce J. Tromberg

University of California, Irvine, Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92612

John V. Frangioni

Beth Israel Deaconess Medical Center, Department of Medicine, Division of Hematology/Oncology, 330 Brookline Avenue, Boston, Massachusetts 02215

J. Biomed. Opt. 14(3), 034045 (June 24, 2009). doi:10.1117/1.3156840
History: Received January 28, 2009; Revised May 06, 2009; Accepted May 06, 2009; Published June 24, 2009
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We describe a noncontact profile correction technique for quantitative, wide-field optical measurement of tissue absorption (μa) and reduced scattering (μs) coefficients, based on geometric correction of the sample’s Lambertian (diffuse) reflectance intensity. Because the projection of structured light onto an object is the basis for both phase-shifting profilometry and modulated imaging, we were able to develop a single instrument capable of performing both techniques. In so doing, the surface of the three-dimensional object could be acquired and used to extract the object’s optical properties. The optical properties of flat polydimethylsiloxane (silicone) phantoms with homogenous tissue-like optical properties were extracted, with and without profilometry correction, after vertical translation and tilting of the phantoms at various angles. Objects having a complex shape, including a hemispheric silicone phantom and human fingers, were acquired and similarly processed, with vascular constriction of a finger being readily detectable through changes in its optical properties. Using profilometry correction, the accuracy of extracted absorption and reduced scattering coefficients improved from two- to ten-fold for surfaces having height variations as much as 3cm and tilt angles as high as 40deg. These data lay the foundation for employing structured light for quantitative imaging during surgery.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Sylvain Gioux ; Amaan Mazhar ; David J. Cuccia ; Anthony J. Durkin ; Bruce J. Tromberg, et al.
"Three-dimensional surface profile intensity correction for spatially modulated imaging", J. Biomed. Opt. 14(3), 034045 (June 24, 2009). ; http://dx.doi.org/10.1117/1.3156840


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