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Research Papers

Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging

[+] Author Affiliations
James J. Mancuso

Baylor College of Medicine, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, One Baylor Plaza, Houston, Texas 77030

Adam M. Larson

Baylor College of Medicine, Department of Neuroscience, One Baylor Plaza, Houston, Texas 77030

Theodore G. Wensel

Baylor College of Medicine, Verna and Marrs McLean Department of Biochemistry and Molecular Biology and Department of Neuroscience, One Baylor Plaza, Houston, Texas 77030

Peter Saggau

Baylor College of Medicine, Department of Neuroscience, One Baylor Plaza, Houston, Texas 77030

J. Biomed. Opt. 14(3), 034048 (December 10, 2008March 11, 2009March 28, 2009May 27, 2009). doi:10.1117/1.3139850
History: Received December 10, 2008; Revised March 11, 2009; Accepted March 28, 2009; Published May 27, 2009
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The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

James J. Mancuso ; Adam M. Larson ; Theodore G. Wensel and Peter Saggau
"Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging", J. Biomed. Opt. 14(3), 034048 (December 10, 2008March 11, 2009March 28, 2009May 27, 2009). ; http://dx.doi.org/10.1117/1.3139850


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