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Research Papers

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

[+] Author Affiliations
Benjamin Rappaz

Ecole Polytechnique Fédérale de Lausanne, Brain Mind Institute, CH-1015 Lausanne, Switzerland

Elena Cano

Ecole Polytechnique Fédérale de Lausanne, ISREC, CH-1015 Lausanne, Switzerland

Tristan Colomb

Lyncée Tec SA, PSE-A, CH-1015 Lausanne, Switzerland

Jonas Kühn, Christian Depeursinge

Ecole Polytechnique Fédérale de Lausanne, Imaging and Applied Optics Institute, CH-1015 Lausanne, Switzerland

Viesturs Simanis

Ecole Polytechnique Fédérale de Lausanne, ISREC, CH-1015 Lausanne, Switzerland

Pierre J. Magistretti

Ecole Polytechnique Fédérale de Lausanne, Brain Mind Institute, CH-1015 Lausanne, Switzerland

Pierre Marquet

Ecole Polytechnique Fédérale de Lausanne, Brain Mind Institute, CH-1015 Lausanne, Switzerland and Centre de neurosciences psychiatriques, Département de psychiatrie DP-CHUV, Site de Cery, CH-1008 Prilly-Lausanne, Switzerland

J. Biomed. Opt. 14(3), 034049 (June 16, 2009). doi:10.1117/1.3147385
History: Received January 22, 2009; Revised March 30, 2009; Accepted March 31, 2009; Published June 16, 2009
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Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Benjamin Rappaz ; Elena Cano ; Tristan Colomb ; Jonas Kühn ; Viesturs Simanis, et al.
"Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy", J. Biomed. Opt. 14(3), 034049 (June 16, 2009). ; http://dx.doi.org/10.1117/1.3147385


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